PERK Antibody
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Catalog numberR31135
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PricePlease ask
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Size0.1mg
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CategoryAntibody
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Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
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FormAntigen affinity purified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenPERK
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB, ICC
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Recommended dilutionsWestern blot: 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
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NotesThe stated application concentrations are suggested starting points. Titration of the PERK antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis PERK antibodyis to be used only for research purposes and not for diagnostics..
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UniprotQ9NZJ5
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PurityAntigen affinity
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DescriptionEukaryotic Translation Initiation Factor 2-Alpha Kinase 3, also called PEK and PERK, is an enzyme encoded by the EIF2AK3 gene. By fluorescence in situ hybridization and radiation hybrid analysis, Hayes et al.(1999) mapped the gene to chromosome 2p12. Harding et al.(2000) reported that a targeted mutation of the mouse Eif2ak3 gene, which they called PERK, abolished the phosphorylation of eIF2-alpha in response to accumulation of malfolded proteins in the ER, resulting in abnormally elevated protein synthesis and higher levels of ER stress. Using a library of endoribonuclease-prepared short interfering RNAs(esiRNAs), Kittler et al.(2004) identified 37 genes required for cell division, one of which was PERK.
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ImmunogenAmino acids 163-176 (QWDQDRESMETVPF) were used as the immunogen for this PERK antibody.
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StorageAfter reconstitution, the PERK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolEIF2AK3
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Short nameAnti-PERK
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameAntibodies to PERK
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameeukaryotic translation initiation factor 2 alpha kinase 3
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-06-14
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data