E. coli Mutant Culture Agar

Description

E. coli Mutant Culture Agar is used to propagate, cultivate and maintain Escherichia coli mutant 113. 3 Dav is ATCC 11105employed in microbiological assay of Vitamin B12.
Other Product
Alternative Name

E.coli Maintenance Medium
Type

Dehydrated Culture Media
Origin

Animal
Classification

Bacterial
Composition g L

Casein enzymic hydrolysate 5.000 Yeast extract 5.000 Liver extract 0.050 Sucrose 12.000 Monopotassium phosphate 0.500 Magnesium sulphate 0.200 Sodium chloride 0.100 Ferrous sulphate 0.001 Agar 15.000 Final pH ( at 25°C) 7.0±0.2
Quantity required per litre

37,85 g
Appearance

Cream to yellow coloured homogeneous free flowing powder
Colour and Clarity of prepared medium

Yellow coloured slightly opalescent gel forms in tubes as slants.
Cultural response

Cultural characteristics observed after an incubation at 35 - 37°C for 18 - 48 hours.
Storage

Store below 30°C in tightly closed container and the prepared medium at 2-8°C.
Shelf Life

Use before expiry date on the label.
Technical Data
Data Sheet

Gene target

Mutant Culture Agar
Short name

Mutant Culture Agar
Technique

culture
Host

e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. Himedia supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
Species

E. coli, E. coli
Alternative name

E. coli Mutant Culture media gelling agent
Alternative technique

escherichia

Name

Electrophoresis, Agar Gel
Concept

Scope note: Electrophoresis in which agar or agarose gel is used as the diffusion medium.
Tree numbers
- E05.196.401.153
- E05.301.300.100
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data