E. coli Fpg FLARE Module
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Catalog number4040-100-FM
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Price:
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Size>100 samples
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Keep at-20° C
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Assay performanceThe FLARE Sample Prep (slides, lysis solution and low melt agarose) are for use with the FLARE Modules, enzyme and buffers for FLARE, Fragment Length Analysis using Repair Enzymes, detecting DNA damage in single cells using E. coli formamidopyrimidine-DNA glycosylase (Fpg) with single cell gel electrophoresis for DNA damage from mutagens, drugs, Fpg catalyzes the excision of the following forms of DNA damage:opening from 7-methylguanine, including 2,6-diamino-4-hydroxy-5-N-methylformamido-pyrimidine, and 4,6-diamino-5-formamidopyimidine, 8- OHdG, 4-HNA or oxoguaninelesions 5-hydroxycytosine and 5-hydroxyuracil, Aflatoxin-bound imidazole-ring-opened guanine, Imidazole ring opened N-2-aminofluorene-C8-guanine. The FLARE slides are then immersed in an alkali solution, to unwind and denature the DNA strands prior to alkali gel electrophoresis.
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More adviceThe FLARE Sample Preparation kit contains the necessary comet assay reagents for preparing your samples for incubation with DNA Repair Enzymes to determine the DNA damage occurred by substrate specificity like by Fpg Comet is used to see the tail length, DNA% in the tail, and tail moment. Trevigen FLARE Slides, in FLARE Assay Kits contain low melting agarose and are not used in diagnostic procedures
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Gene target
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Short nameFpg FLARE Module
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Hoste. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. Trevigen supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
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SpeciesE. coli, E. coli
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Alternative nameE. coli Fpg FLARE Module
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Alternative techniqueescherichia
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