Recombinant Mouse SRC Kinase / Proto-oncogene c-Src Protein (His & GST tag)
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Catalog number
PKSM040305-20µg
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Price
Please ask
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Size
20µg
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Synonym
AW259666;pp60c-src
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Activity
1. The specific activity was determined to be >80 nmol/min/mg using poly [Glu, Tyr] 4:1 as substrate.2. Measured by its binding ability in a functional ELISA. Immobilized recombinant Mouse SRC at 2 μg/ml (100 μl/well) can bind biotinylated human PTPRA (aa 174-793) with a linear range of 0.032-0.8 μg/ml.
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Sequence
Met 1-Leu 535
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Fusion tag
N-His & GST
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Accession
NP_001020566.111
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Expressed Host
Baculovirus-Insect Cells
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Shipping
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.Shipment made at ambient temperature may seriously affect the activity of the ordered products.
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Purity
> 95 % as determined by SDS-PAGE
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Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
87.7 kDa
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AP Mol Mass
80 kDa
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Formulation
Supplied as sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0
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Background
Proto-oncogene tyrosine-protein kinase SRC is a hydrophobic protein belonging to the SRC family kinase including nine members that is a family of non-receptor tyrosine kinases. SRC protein may exist in different forms: C-SRC and V-SRC. C-SRC is only activated under certain circumstances where it is required such as growth factor signaling, while V-SRC is a constitutively active as opposed to normal SRC (C-SRC). Thus, V-SRC is an instructive example of an oncogene protein kinase whereas C-SRC is a proto-oncogene protein kinase. Inhibition of SRC with NR2A tyrosine phosphorylation mediated by PSD-95 may contribute to the lithium-induced downregulation of NMDA receptor function and provide neuroprotection against excitotoxicity.
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Conjugation
histidine
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
SRC, MGST3, NCOA1, NCOA3
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Short name
Recombinant Mouse SRC Kinase / Proto-oncogene c-Src Protein (His & GST tag)
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Technique
Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Host
mouse
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Label
His
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Species
Mouse, Mouses
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Alternative name
Rec. Mouse v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) phosphorylation catalyst / Proto-oncogene c-v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) Protein (histidine & GST detection labelled)
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Alternative technique
rec, murine
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Alternative to gene target
v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian), ASV and c-SRC and p60-Src and SRC1, SRC and IDBG-74081 and ENSG00000197122 and 6714, transferase activity, nuclei, Src and IDBG-211273 and ENSMUSG00000027646 and 20779, BT.61976 and IDBG-642561 and ENSBTAG00000008938 and 535742
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Gene info
Gene info
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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