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Stock availability
Available
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Category
Secondary Antibodies
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Antibody type
Polyclonal Antibody
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Antibody host
Donkey
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Antibody conjugate
HRP
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Technical datasheet
Contact Gentaur to request the datasheet or ask our specialists for more information.
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Notes
The Donkey Anti-Human IgG (H+L)-HRP Conjugate (adsorbed with bovine, chicken, goat, G. pig, hamster, horse, mouse, rabbit, rat, and sheep) is manufactured for Research Use Only or for diagnostics purposes.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
adi supplies antibodies that are for research of human proteins. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Properties
Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken. Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry. Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Latin name
Equus africanus asinus, Capra aegagrus hircus, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus
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Conjugation
HRP Conjugated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Pigs and the smaller guinea pigs are frequent used as models for humans. Rabbits are used for polyclonal antibody production by adi. Rabbit antibodies are very stable and can be stored for several days at room temperature. adi adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target
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Short name
Anti- IgG (H+L)- Conjugate (adsorbed with , , goat, G. , , , mouse, rabbit, , )
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Technique
anti-Human, Rabbit, anti-, Mouse, anti, Goat, IgG, antibody to, antibodies, antibodies against human proteins, goats, IgGs, mouses
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Host
Goat, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits. The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
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Isotype
IgG, IgG
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Label
HRP
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Species
Human, Bos Taurus genes are available form the Bovine Genome database. Chickens, Donkeys, Goats, Hamsters, Horses, Humans, Mouses, Pigs and Guinea pigs, Rats, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
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Alternative name
Donkey Antibody toHuman Immunoglobulin G (heavy + light chain)-horseradish peroxidase Conjugate (adsorbed including bovine, chicken, caprine, G. swine, hamster, horse, mouse, production species: rabbit, rat, and ovine)
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Alternative technique
rabbit-anti, murine, antibodies, caprine, immunoglobulins
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