DARC Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

DNA lentivector for transduction information

Lentiveral packaging plasmid DNA for non-viral plasmid transfection and direct use in plasmid expression. This DNA can alos be used for packaging into Lentiviral particles for high efficiency transduction and stably integrated expressions. GENTAUR suggests to use our ABM packaging mix LV003 of second generation virusses or the LV053, our 3rd Generation Packaging mixture. pLenti lentiviral plasmids DNAs are stored in 10milliMolar Tris/HCI with 1mM EDTA at a pH of 8 at -25 C. Vectors with selection markers like kanamycin, puromycin or cumate are available.
Lentiviral technical files
Lentivirus references
Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Gene target

DARC Lentiviral Vector CMV pLenti-GIII-CMV-RFP-2A-Puro
Gene symbol

TRIM27, IRX5, PLA2G3, IGHD2OR15-2A, EIF2A, CXCL2, RNVU1-2A, PLPP1, CHD3, ACKR1
Short name

DARC Lentiviral Vector ( ) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)
Technique

Vectors
Label

puro
Species

Human, Humans
Alternative name

Duffy blood group, chemokine receptor Lentiviral integrating Desoxyribonucleic acid sequence (H. sapiens) (cytomegalovirus) (pLenti-GIII-cytomegalovirus-Red Fluorescent-2A-Puro)
Alternative to gene target

Duffy blood group, chemokine receptor, CCBP1 and CD234 and DARC and Dfy and FY and GPD and GpFy and WBCQ1, DARC and IDBG-246055 and ENSG00000213088 and 2532, C-C chemokine binding, Plasma membranes, Darc and IDBG-205828 and ENSMUSG00000037872 and 13349, BT.27077 and IDBG-630777 and ENSBTAG00000003220 and 528076
Virus

cmv

Identity

HGNC:1918
Gene

CHD3
Long gene name

chromodomain helicase DNA binding protein 3
Synonyms
- Mi-2a
- ZFH
- Mi2-ALPHA
GenBank acession
- U08379
Locus

17p13.1
Discovery year

1998-03-20
Entrez gene record

1107
Pubmed identfication
- 9326634
- 7560064
RefSeq identity
- NM_001005273
Classification
- Small nucleolar RNA protein coding host genes
- NuRD complex subunits
- PHD finger proteins
VEGA ID

OTTHUMG00000150427

Identity

HGNC:4035
Gene

ACKR1
Long gene name

atypical chemokine receptor 1 (Duffy blood group)
Synonyms gene
- FY
- DARC
Synonyms gene name
- Duffy blood group
- Duffy blood group, chemokine receptor
- Duffy blood group, atypical chemokine receptor
Synonyms
- CCBP1
- GPD
- Dfy
- CD234
Synonyms name
- atypical chemokine receptor 1
- glycoprotein D
GenBank acession
- U01839
Locus

1q23.2
Discovery year

2001-06-22
Entrez gene record

2532
Pubmed identfication
- 8248172
- 7663520
- 16148
RefSeq identity
- NM_002036
Classification
- Atypical chemokine receptors
- Blood group antigens
- CD molecules
VEGA ID

OTTHUMG00000037182
Locus Specific Databases
- LRG_801

Name

Virus Cultivation
Concept

Scope note: Process of growing viruses in live animals, plants, or cultured cells.
Tree numbers
- E01.370.225.875.970
- E05.200.875.970
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data