Cytokeratin 14 Antibody (CK14, KRT14)

• Catalog number
  R32606
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Cytokeratin 14 (CK14, KRT14)
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Notes
  Optimal dilution of the Cytokeratin 14 antibody should be determined by the researcher.
• Added buffer
  Lyophilized from 1X Phosphate Buffered Saline (PBS) with 2.5% BSA and 0.025% sodium azide
• Intented use
  This antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P02533
• Purity
  Antigen affinity
• Description
  Cytokeratin 14, also known as cytokeratin-14 (CK-14) or keratin-14 (KRT14), is a member of the type I keratin family of intermediate filament proteins. In humans it is encoded by the KRT14 gene. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.
• Immunogen
  Amino acids 446-472 (RQIRTKVMDVHDGKVVSTHEQVLRTKN-human) were used as the immunogen for the Cytokeratin 14 antibody.
• Storage
  After reconstitution, the Cytokeratin 14 antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Localization
  Cytoplasmic, nuclear
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Cytokeratin   CK14   KRT14  
• Gene symbol
  KRT14
• Short name
  Anti- Cytokeratin 14 (CK14, KRT14)
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to Cytokeratin 14 (CK14, KRT14)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:6416
• Gene
  KRT14
• Long gene name
  keratin 14
• Synonyms gene
  • EBS3
  • EBS4
• Synonyms gene name
  • keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
  • keratin 14, type I
• Synonyms name
  • epidermolysis bullosa simplex, Dowling-Meara, Koebner
• GenBank acession
  • BC002690
• Locus
  17q21.2
• Discovery year
  1992-04-09
• Entrez gene record
  3861
• Pubmed identfication
  • 1717157
  • 16831889
• RefSeq identity
  • NM_000526
• Classification
  • Keratins, type I
• VEGA ID
  OTTHUMG00000133426

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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