Mouse Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial ELISA Kit
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Catalog numberEK3579
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PricePlease ask
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Size96-wells plate (breakable strips)
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Long nameELISA test kit for Mouse Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
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Synonym nameMurine Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial ELISA Kit
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General descriptionThis is a ready to use kit for performing ELISA tests for detection of Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial in samples from Mouse
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AntigenDelta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
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Species reactivityMouse
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Sample typesOur Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial ELISA kit has been proven to work and successfully detect Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial in Murine
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Kit contentsELISA test kit for Mouse Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrialAssay plate 1 (96wells); Standard 5 x 0.5 ml; A ntibody 1x5ml; Conjugate 1x10ml; Wash buffer 20ml (25X); Substrate A 1x5ml; Substrate B 1x5ml; Stop solution 1x5ml;Adhesive strips (4pcs); working manual
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Detection wavelengt450 nm
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Assay procedure1-4h
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Summary of the prodecure1. Prepare the reagents and samples according to the manual; 2. Leave one well empy; 3. Place 50ul of standard or sample in each well; 4. Add 50ul of the antibody to each well except the Blank (empty); 5 Allow 60 minutes for incubation at 37 degrees celsius; 6. Wash the wells 5 times; 7. Add 100ul of the conjugate to each well but the Blank one; 8. Allow 30 more minutes for incubation at 37C; 9. Wash 5 more times; 10. Add 50ul of both Substrate A and Substrate B in each well (incl. the Blank) and incubate for 15 minutes; 11. Put 50 ul of Stop Solution in all wells (incl. the Blank) and read the results within maximum 10 minutes; Notes: *Samples might require dilution, follow the sample dilution instructions of the manual. **During the last incubation with Substrates A and B the setting should be protected from any light.
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ApplicationsEnzyme Linked Immunosorbent Assay
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Shipping storage shelf lifeThe Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial ELISA kit is shipped on ice packs. Upon delivery store the kit at +4 C. The shelf life of the Mouse Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial ELISA Kit is 6 months after delivery.
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TipsDue to transportation and handling small amounts of the liquid components of the Murine Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial ELISA Kit can get caught on the walls or lid of the vial. Prior to use be sure to briefly centrifuge all vials to ensure that all of the content is on the bottom. Do not freeze the kit, as this might denaturate the standards and the peptide chains of the antibodies used for the production if the ELISA kit. Substrate A and Substrate B are light sensitive, therefore you should protect the kits from direct light. When using the vials of Substrates A and B you may cover them in aluminium foil to protect them from light influences.
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PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
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TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolIGHVIII-5-1, TRE-CTC1-5, TRL-CAG1-5, TRG-GCC1-5, CYB5AP3, TRUND-NNN5-1, TEAD3, TRV-CAC1-5, TRA-TGC5-1, TRQ-CTG5-1
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Short nameDelta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial ELISA
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TechniqueELISA kit, ELISA, Mouse, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests. mouses
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Hostmouse, antigen assay ELISA kit 1 Kit (96 Wells)
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SpeciesMouse, Mouses
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Alternative nameMouse Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial Enzyme-linked immunosorbent assay reagent
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Alternative techniquemurine, elisas, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissuemitochondrial
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Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (III)-5-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (III)-5-1
- immunoglobulin heavy variable (III)-5-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametRNA-Glu (anticodon CTC) 1-5
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Synonyms gene
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Synonyms gene name
- transfer RNA glutamic acid 5 (anticodon CUC)
- transfer RNA-Glu (CTC) 1-5
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Leu (anticodon CAG) 1-5
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Synonyms gene
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Synonyms gene name
- transfer RNA leucine 14 (anticodon CAG)
- transfer RNA-Leu (CAG) 1-5
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Gly (anticodon GCC) 1-5
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Synonyms gene
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Synonyms gene name
- transfer RNA glycine 27 (anticodon GCC)
- transfer RNA-Gly (GCC) 1-5
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene namecytochrome b5 type A pseudogene 3
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Synonyms gene
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Synonyms gene name
- cytochrome b-5 pseudogene 3
- cytochrome b5 type A (microsomal) pseudogene 3
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Synonyms
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GenBank acession
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Locus
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Discovery year1994-07-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 5-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 5-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
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Classification
- Low confidence cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nameTEA domain transcription factor 3
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Synonyms gene
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Synonyms gene name
- TEA domain family member 3
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-08-10
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Entrez gene record
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Pubmed identfication
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Classification
- TEA domain transcription factors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametRNA-Val (anticodon CAC) 1-5
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Synonyms gene
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Synonyms gene name
- transfer RNA valine 6 (anticodon CAC)
- transfer RNA-Val (CAC) 1-5
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Synonyms
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GenBank acession
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Locus
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Discovery year2007-01-11
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Entrez gene record
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Pubmed identfication
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Ala (anticodon TGC) 5-1
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Synonyms gene
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Synonyms gene name
- transfer RNA alanine 10 (anticodon UGC)
- transfer RNA-Ala (TGC) 5-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Gln (anticodon CTG) 5-1
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Synonyms gene
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Synonyms gene name
- transfer RNA glutamine 27 (anticodon CUG)
- transfer RNA-Gln (CTG) 5-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
MeSH Data
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Name
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ConceptScope note: Radioimmunoassay of proteins using antibody coupled to an immunosorbent.
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Tree numbers
- E05.478.566.380.830
- E05.478.566.639.830
- E05.601.470.380.830
- E05.601.470.639.830
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Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
