CSTB, human recombinant

• Catalog number
  9235-25
• Price
  Please ask
• Size
  25 µg

• Synonyms
  Cystatin-B, Stefin B, PME, CST7
• Alternative_names
  Cystatin-B, Stefin B, PME, CST7
• Description
  An anti-protease implicated in myoclonus epilepsy.
• Recombinant
  Yes
• Source
  E.coli
• Purity by SDS PAGE
  >95%
• Assay
  SDS-PAGE
• Purity
  >95%
• Biological activity
  IC50 value is < 3nM
• Molecular Weight
  This protein is fused with a 6× His tag at N-terminus and has a calculated MW of 13 kDa.
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  Liquid
• Reconstitution Instructions
  In 20 mM Tris-HCl buffer (pH8.0) containing 50mM NaCl.
• Background Information
  CSTB, also known as Cystatin B is an anti-protease implicated in myoclonus epilepsy, a degenerative disease of the central nervous system. The cystatin superfamily encompasses proteins that contain multiple cystatin like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. This protein is able to form a dimer stabilized by noncovalent forces and is thought to play a role in protecting against the proteases leaking from lysosomes. In cells, CSTB is located in the lysosomes and the cytoplasm, but also in the nucleus.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MMCGAPSATQ PATAETQHIA DQVRSQLEEK ENKKFPVFKA VSFKSQVVAG TNYFIKVHVG DEDFVHLRVF QSLPHENKPL TLSNYQTNKA KHDELTYF
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CSTB  
• Gene symbol
  CSTB
• Short name
  CSTB, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  cystatin B (stefin B), H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  cystatin B (stefin B), CST6 and EPM1 and EPM1A and PME and STFB and ULD, CSTB and IDBG-4888 and ENSG00000160213 and 1476, poly(A) RNA binding, nuclei, Cstb and IDBG-169100 and ENSMUSG00000005054 and 13014

Gene info

• Identity
  HGNC:2482
• Gene
  CSTB
• Long gene name
  cystatin B
• Synonyms gene
  • EPM1
  • STFB
• Synonyms gene name
  • cystatin B (stefin B)
• Synonyms
  • CST6
  • PME
• Synonyms name
  • stefin B
  • Epilepsy, progressive myoclonic 1
• GenBank acession
  • L03558
• Locus
  21q22.3
• Discovery year
  1996-12-12
• Entrez gene record
  1476
• Pubmed identfication
  • 8596935
• RefSeq identity
  • NM_000100
• Classification
  • Cystatins, type 1
• VEGA ID
  OTTHUMG00000086886
• Locus Specific Databases
  • LRG_485

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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