Calponin Antibody

• Catalog number
  R31348
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Calponin
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the Calponin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This Calponin antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P51911
• Purity
  Antigen affinity
• Description
  Calponin is a basic 34-kD protein first isolated from chicken gizzard and bovine aorta. This gene is mapped to 19p13.2. It is specifically expressed in smooth muscle and binds calmodulin, actin, and tropomyosin. It is able to inhibit the ATPase activity of myosin and is though to play a role in smooth muscle contraction. Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein though to be involved in some way in the regulation or modulation of contraction.
• Immunogen
  An amino acid sequence from the C-terminus of human Calponin (EPAHNHHAHNYYNSA) was used as the immunogen for this Calponin antibody.
• Storage
  After reconstitution, the Calponin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Calponin  
• Gene symbol
  CNN3, CNN3P1, CNN2P8
• Short name
  Anti-Calponin
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to Calponin
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:2157
• Gene
  CNN3
• Long gene name
  calponin 3
• Synonyms gene name
  • calponin 3, acidic
• GenBank acession
  • BC025372
• Locus
  1p21.3
• Discovery year
  1996-08-16
• Entrez gene record
  1266
• Pubmed identfication
  • 8526917
• RefSeq identity
  • NM_001839
• VEGA ID
  OTTHUMG00000010783

Gene info

• Identity
  HGNC:38666
• Gene
  CNN3P1
• Long gene name
  calponin 3 pseudogene 1
• Synonyms gene name
  • calponin 3, acidic pseudogene 1
• Locus
  6p25.1
• Discovery year
  2010-07-08
• Entrez gene record
  643875
• VEGA ID
  OTTHUMG00000014189

Gene info

• Identity
  HGNC:39533
• Gene
  CNN2P8
• Long gene name
  calponin 2 pseudogene 8
• Locus
  2q11.1
• Discovery year
  2011-02-18
• Entrez gene record
  205272
• VEGA ID
  OTTHUMG00000155130

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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