Chicken, IgG and serum proteins

• Catalog number
  AS12 2301
• Price
  Please ask
• Size
  1 mg

• Available ordering format
  Lyophilized
• Immunogen
  Purified Mouse IgG, whole molecule
• Raised in
  Chicken
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified Chicken IgY
• How to reconstitute
  For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
• Storage condition
  Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
• Verified applications
  Contact Gentaur support center
• Connected products
  other dylight anti-Mouse secondary antibodies
• Recommended dilutions for use
  1:20 - 1:2,000 for most applications
• Molecular weight expected аpparent
  see datasheet
• Verified reactivity
  to be determined
• Possible reactivity
  to be determined
• No reactivity
  to be determined
• Supplementary information
  Contact Gentaur support center at [email protected]
• References
  to be added when available
• Scientific context
  Chicken anti-Mouse IgG (H&L) - DyLight®650 Conjugated, min. cross-reactivity to human or rabbit IgG and serum proteins is a secondary antibody conjugated to DyLight® 650, which binds to Mouse IgG (H&L) in immunological assays.DyLight® 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are purified using solid phase Mouse IgG (H&L)DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
• Notes
  Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (gamma) chains on mouse IgG light chains on all mouse immunoglobulinsNo reactivity is observed to: non-immunoglobulin mouse serum proteins, light chains on all human immunoglobulins, IgG from human or rabbit serum proteins from human or rabbit
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Properties
  Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Serum should be stored at +5°C
• Description
  Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
• Group
  Sera

• Gene target
  proteins  
• Gene symbol
  PGAP1, PGAP3, MPPE1
• Short name
  , IgG serum proteins
• Technique
  serum, IgG, IgGs
• Isotype
  IgG, IgG
• Species
  chicken, Chickens
• Alternative name
  Chicken, Immunoglobulin G and blood serum proteins
• Alternative technique
  sera, immunoglobulins
• Tissue
  serum

Gene info

• Identity
  HGNC:25712
• Gene
  PGAP1
• Long gene name
  post-GPI attachment to proteins inositol deacylase 1
• Synonyms gene name
  • post-GPI attachment to proteins 1
• Synonyms
  • FLJ12377
  • Bst1
  • SPG67
• Synonyms name
  • GPI inositol-deacylase
• Locus
  2q33.1
• Discovery year
  2008-02-26
• Entrez gene record
  80055
• Pubmed identfication
  • 14734546
  • 17711852
  • 24482476
• RefSeq identity
  • NM_024989
• VEGA ID
  OTTHUMG00000132743

Gene info

• Identity
  HGNC:23719
• Gene
  PGAP3
• Long gene name
  post-GPI attachment to proteins phospholipase 3
• Synonyms gene
  • PERLD1
• Synonyms gene name
  • per1-like domain containing 1
  • post-GPI attachment to proteins 3
• Synonyms
  • MGC9753
  • CAB2
  • PP1498
  • PER1
• Synonyms name
  • post-GPI attachment to proteins 3
• GenBank acession
  • AB088396
• Locus
  17q12
• Discovery year
  2004-04-30
• Entrez gene record
  93210
• Pubmed identfication
  • 15010812
  • 17021251
  • 17314402
• RefSeq identity
  • NM_033419
• VEGA ID
  OTTHUMG00000179110

Gene info

• Identity
  HGNC:15988
• Gene
  MPPE1
• Long gene name
  metallophosphoesterase 1
• Synonyms
  • PGAP5
  • Cdc1
• Synonyms name
  • post-GPI attachment to proteins 5
• GenBank acession
  • BC002877
• Locus
  18p11.21
• Discovery year
  2003-10-17
• Entrez gene record
  65258
• Pubmed identfication
  • 11978971
  • 19837036
• RefSeq identity
  • NM_023075
• VEGA ID
  OTTHUMG00000131661

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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