Checkpoint Kinase Activity Immunoblot test kit
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Catalog number
STA-413
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Price
Please ask
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Size
20 assays
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We send this reagant on
Blue Ice
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The temperatureerature for storage is
Store active CHK1 at -80º C and all other test kit components at -20º C. Avoid multiple freeze/thaw cycles.
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Technical file
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SDS or safty data sheet
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Type of reagent
Cell Signaling and Protein Biology
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Related techniques
Cell Signaling and Protein Biology, Kinase Assays, Checkpoint Kinase Activity Assays
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Use of the reagent
Cell Biolabs’ Checkpoint Kinase Activity Immunoblot test kit utilizes recombinant Cdc25C as checkpoint kinase substrate. After incubating the substrate with checkpoint kinase samples (such as purified kinase, cell lysate or immunoprecipitate), the phosphorylated Cdc25C is detected by western blot analysis using an anti-phospho-Cdc25C (Ser216).
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Cellular use
Simple and fast tool to monitor checkpoint kinase activity using its physiological substrate
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Protocol information
Suitable for use with samples such as purified kinase or cell lysate
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Species compatible to
Humans
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Use of this test kit or product
Genetics lab
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Antigen
a specific protein sequence
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Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,92
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Molecular weight
in kilo Danlto or kDa
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This reagent is purified
0.97
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Gene target
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Short name
Checkpoint Kinase Activity Immunoblot test kit
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Alternative name
Checkpoint phosphorylation catalyst Activity Immunoblot test reagent
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Alternative technique
tests, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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