CGL78, YCF54
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Catalog numberAS10 936
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Price:
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Size200 µl
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Available ordering formatLyophilized
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Immunogenrecombinant fragment of Arabidopsis thaliana CGL78 Q9LVM3 (At5g58250)
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Raised inRabbit
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ClonalityPolyclonal
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ClonePolyclonal
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PurificationSerum
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How to reconstituteFor reconstitution add 200 µl of sterile water.
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Storage conditionstore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applicationswestern blot (WB)
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Connected productsAS06 122 | CRD1 | cyanobacterial homolog of plant CHL27 cyclasePlant and algal protein extraction buffer Secondary antibodies
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Recommended dilutions for use1 : 1000 with standard ECL (WB)
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Molecular weight expected аpparent24 kDa
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Verified reactivityArabidopsis thaliana , Chlamydomonas reinhardtii, Synechocystis sp. PCC 6803
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Possible reactivityn.a.
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No reactivityHordeum vulgare
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Supplementary informationPlease, omitt SDS from transfer buffer and reduce transfer time to 45 min. Nitrocellulose membrane is recommended and SDS is omitted to allow this LMW protein to bind tighter to the membrane.
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ReferencesHsieh et al. (2013). The Proteome of Copper, Iron, Zinc, and Manganese Micronutrient Deficiency in Chlamydomonas reinhardtii. Mol Cell Proteomics. 2013 Jan;12(1):65-86. doi: 10.1074/mcp.M112.021840. Epub 2012 Oct 13.
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Scientific contextCGL78 is a protein conserved in the green lineage and predicted to be located in the chloroplast. Member of the Ycf54 superfamily.
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NotesCurrently this antibody has not been confirmed to detect CGL78 protein in Arabidopsis thaliana. If you are interested to use this antibody in Arabidopsis, please, contact us.
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Protein numberQ9LVM3
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TAIR numberAT5G58250
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Gene target
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Short nameCGL78, YCF54
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Alternative nameCGL78, YCF54
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