CDC5L Lentiviral Vector (mouse) (pLenti-III-HA/mKate2-N-term)

DNA lentivector for transduction information

Lentiveral packaging plasmid DNA for non-viral plasmid transfection and direct use in plasmid expression. This DNA can alos be used for packaging into Lentiviral particles for high efficiency transduction and stably integrated expressions. GENTAUR suggests to use our ABM packaging mix LV003 of second generation virusses or the LV053, our 3rd Generation Packaging mixture. pLenti lentiviral plasmids DNAs are stored in 10milliMolar Tris/HCI with 1mM EDTA at a pH of 8 at -25 C. Vectors with selection markers like kanamycin, puromycin or cumate are available.
Lentiviral technical files
Lentivirus references
Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Latin name

Mus musculus

Gene target

CDC5L Lentiviral Vector pLenti-III-HA/mKate2-N-term
Gene symbol

GH1, POLM, NRAS, SDC3, PIGN, WASL, MAP6, HLA-N, FERD3L, SHC3
Short name

CDC5L Lentiviral Vector (mouse) (pLenti-III-HA/mKate2-N-term)
Technique

Mouse, mouses, Vectors
Host

mouse
Species

Mouse, Mouses
Alternative name

CDC5L Lentiviral integrating Desoxyribonucleic acid sequence (mouse) (pLenti-III-HA/mKate2-n terminus)
Alternative technique

murine

Identity

HGNC:7989
Gene

NRAS
Long gene name

NRAS proto-oncogene, GTPase
Synonyms gene name
- neuroblastoma RAS viral (v-ras) oncogene homolog
- neuroblastoma RAS viral oncogene homolog
Synonyms
- N-ras
GenBank acession
- BC005219
Locus

1p13.2
Discovery year

2001-06-22
Entrez gene record

4893
Pubmed identfication
- 6705568
- 11524732
RefSeq identity
- NM_002524
Classification
- RAS type GTPase family
VEGA ID

OTTHUMG00000012059
Locus Specific Databases
- NRASbase: Mutation registry for autoimmune lymphoproliferative syndrome type IV
- LRG_92

Identity

HGNC:12735
Gene

WASL
Long gene name

WASP like actin nucleation promoting factor
Synonyms gene name
- Wiskott-Aldrich syndrome-like
- Wiskott-Aldrich syndrome like
Synonyms
- N-WASP
- NWASP
- WASPB
GenBank acession
- D88460
Locus

7q31.32
Discovery year

1998-10-14
Entrez gene record

8976
Pubmed identfication
- 9422512
- 9322739
- 23527602
RefSeq identity
- NM_003941
Classification
- Wiskott-Aldrich Syndrome protein family
VEGA ID

OTTHUMG00000157346

Identity

HGNC:16660
Gene

FERD3L
Long gene name

Fer3 like bHLH transcription factor
Synonyms gene name
- Fer3-like (Drosophila)
- Fer3-like bHLH transcription factor
Synonyms
- NATO3
- N-TWIST
- bHLHa31
GenBank acession
- AF369897
Locus

7p21.1
Discovery year

2002-12-09
Entrez gene record

222894
Pubmed identfication
- 11472856
- 12217327
Classification
- Basic helix-loop-helix proteins
VEGA ID

OTTHUMG00000090823

Identity

HGNC:18181
Gene

SHC3
Long gene name

SHC adaptor protein 3
Synonyms gene name
- src homology 2 domain containing transforming protein C3
- SHC (Src homology 2 domain containing) transforming protein 3
Synonyms
- N-Shc
- NSHC
- SHCC
GenBank acession
- D84361
Locus

9q22.1
Discovery year

2003-11-12
Entrez gene record

53358
Pubmed identfication
- 8808684
RefSeq identity
- NM_016848
Classification
- SH2 domain containing
VEGA ID

OTTHUMG00000020179

Name

Artificial Gene Fusion
Concept

Scope note: The in vitro fusion of GENES by RECOMBINANT DNA techniques to analyze protein behavior or GENE EXPRESSION REGULATION, or to merge protein functions for specific medical or industrial uses.
Tree numbers
- E05.393.220.250
Qualifiers

drug effects, methods, radiation effects