CD8.1, Lyt-2.1 (allele-specific), Clone: CD8-E1, Mouse Monoclonal antibody-Mouse, Biotin
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Catalog numberDEV102-1-3/1
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PricePlease ask
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Size1 mg
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Alternative name 1CD8.1, Lyt-2.1 (allele-specific), Clone: CD8-E1, Mab anti-Mouse, Biotin
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Alternative name 2CD8.1, Lyt-2.1 (allele-specific), Clone: CD8-E1, Mab antibody-Mouse, Biotin
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Alternative name 3CD8.1, Lyt-2.1 (allele-specific), Clone: CD8-E1, Mab antibody-Mus musculus, Biotin
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Alternative name 4anti-CD8.1 Lyt-2.1 allele-s cic Clone: CD8-E1
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Alternative name 5CD8.1 Lyt-2.1 allele-s cic Clone: CD8-E1
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ClonalityMonoclonal Antibody
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DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
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Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
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AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
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PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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ConjugationBiotinylated
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Additional descriptionCytotoxic T cells cluster differentiators for flow cytometry. This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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French translationanticorps
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Gene target
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Gene symbolCD8A, RNU105C, SNORA73A, RNU105B, ENOPH1, BCHE, ERVE-1, GATA1, MAGED4, OGDH
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Short nameCD8 1, Lyt-2 1 (allele-specific), Clone: CD8-E1, Mouse Monoclonal antibody-Mouse,
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TechniqueMouse monoclonal, Antibody, Mouse, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
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Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
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LabelBiotin
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SpeciesMouse, Mouses
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Alternative nameCD8.1, Lyt-2.1 (allele-specific), clonality: CD8-E1, Mouse monoclonal (antibody to-)-Mouse, biotinilated
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Alternative techniqueantibodies, murine
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Gene info
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Identity
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Gene
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Long gene nameCD8a molecule
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Synonyms gene
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Synonyms gene name
- CD8 antigen, alpha polypeptide (p32)
- T-cell surface glycoprotein CD8 alpha chain
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- V-set domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameRNA, U105C small nucleolar
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-12-14
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene namesmall nucleolar RNA, H/ACA box 73A
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Synonyms gene
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Synonyms gene name
- RNA, U17a small nucleolar
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-08-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Small nucleolar RNAs, H/ACA box
Gene info
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Identity
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Gene
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Long gene nameRNA, U105B small nucleolar
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-12-14
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene nameenolase-phosphatase 1
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Synonyms
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Synonyms name
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Locus
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Discovery year2007-01-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- HAD Asp-based non-protein phosphatases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namebutyrylcholinesterase
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Synonyms gene
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Synonyms gene name
- cholinesterase 1
- cholinesterase (serum) 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameendogenous retrovirus group E member 1
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Synonyms gene
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Synonyms gene name
- endogenous retroviral sequence E, 1
- endogenous retrovirus group E, member 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-04
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameGATA binding protein 1
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Synonyms gene
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Synonyms gene name
- GATA-binding protein 1 (globin transcription factor 1)
- globin transcription factor 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1990-09-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- GATA zinc finger domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameMAGE family member D4
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Synonyms gene name
- melanoma antigen family D4
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-12-23
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MAGE family
- Small nucleolar RNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameoxoglutarate dehydrogenase
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Synonyms gene name
- oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
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Synonyms
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GenBank acession
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Locus
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Discovery year1989-06-30
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Entrez gene record
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Pubmed identfication
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Classification
- Oxoglutarate dehydrogenase family
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data