CD3/CD14/CD16/CD19/CD34, Dendritic Cell Exclusion cocktail, Clone cocktail: 54.1/TUK4/3G8/SJ25-C1/581, Mouse Monoclonal antibody-Human, RPE-Cy5; flow

• Catalog number
  YSRTMCA2248C
• Price
  Please ask
• Size
  1000ul

• Alternative name 1
  CD3/CD14/CD16/CD19/CD34, Dendritic Cell Exclusion cocktail, Clone cocktail: 54.1/TUK4/3G8/SJ25-C1/581, Mab anti-Human, RPE-Cy5; flow
• Alternative name 2
  CD3/CD14/CD16/CD19/CD34, Dendritic Cell Exclusion cocktail, Clone cocktail: 54.1/TUK4/3G8/SJ25-C1/581, Mab antibody-Human, RPE-Cy5; flow
• Alternative name 3
  CD3/CD14/CD16/CD19/CD34, Dendritic Cell Exclusion cocktail, Clone cocktail: 54.1/TUK4/3G8/SJ25-C1/581, Mab antibody-Homo sapiens, RPE-Cy5; flow
• Alternative name 4
  anti-CD3/CD14/CD16/CD19/CD34 Dendritic Cell Exclusion cocktail Clone cocktail: 54.1/TUK4/3G8/SJ25-C1/581 flow
• Alternative name 5
  CD3/CD14/CD16/CD19/CD34 Dendritic Cell Exclusion cocktail Clone cocktail: 54.1/TUK4/3G8/SJ25-C1/581 flow
• Clonality
  Monoclonal Antibody
• Description
  This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
• Storage and handling
  Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
• Advisory
  To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
• Properties
  If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Stem cell or hematopoietic marker. The most used affinity clone is QBEND10. We compare the affinity to this clone. Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional description
  For cells, cell lines and tissues in culture till half confluency.
• Conjugation
  Cy5.5
• Test
  Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• Latin name
  Mus musculus
• French translation
  anticorps

• Gene target
  CD3/CD14/CD16/CD19/CD34   Dendritic   Exclusion   cocktail   cocktail   1/TUK4/3G8/SJ25-C1/581   Monoclonal   RPE  
• Gene symbol
  RPE, CD34, CD19, CD14, SNAR-C1, TRGC1, PFDN4, TNS2, ARHGAP4, MAGEC1
• Short name
  CD3/CD14/CD16/CD19/CD34, Dendritic Exclusion cocktail, Clone cocktail: 54 1/TUK4/3G8/SJ25-C1/581, Mouse Monoclonal antibody- , RPE- ;
• Technique
  Mouse monoclonal, Antibody, Mouse, Flow, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
• Host
  mouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
• Label
  rpe
• Species
  Human, Humans, Mouses
• Alternative name
  CD3/CD14 molecule/CD16/CD19 molecule/CD34 molecule, Dendritic cellular Exclusion cocktail, clonality cocktail: 54.1/TUK4/3G8/SJ25-C1/581, Mouse monoclonal (antibody to-)-H. sapiens, RPE-cyanine 5; flow
• Alternative technique
  antibodies, murine
• Alternative to gene target
  CD19 molecule, B4 and CVID3, CD19 and IDBG-23791 and ENSG00000177455 and 930, protein binding, Cell surfaces, Cd19 and IDBG-209475 and ENSMUSG00000030724 and 12478, CD19 and IDBG-638894 and ENSBTAG00000032122 and
• Clone name
  cocktail
• Tissue
  cell, dendritic

Gene info

• Identity
  HGNC:10293
• Gene
  RPE
• Long gene name
  ribulose-5-phosphate-3-epimerase
• Locus
  2q34
• Discovery year
  2001-06-22
• Entrez gene record
  6120
• RefSeq identity
  • NM_006916
• VEGA ID
  OTTHUMG00000154690

Gene info

• Identity
  HGNC:1662
• Gene
  CD34
• Long gene name
  CD34 molecule
• Synonyms gene name
  • CD34 antigen
• GenBank acession
  • M81104
• Locus
  1q32.2
• Discovery year
  1990-09-10
• Entrez gene record
  947
• Pubmed identfication
  • 1370171
  • 1374051
• RefSeq identity
  • NM_001773
• Classification
  • CD molecules
• VEGA ID
  OTTHUMG00000036565

Gene info

• Identity
  HGNC:1633
• Gene
  CD19
• Long gene name
  CD19 molecule
• Synonyms gene name
  • CD19 antigen
• Locus
  16p11.2
• Discovery year
  1991-06-04
• Entrez gene record
  930
• Classification
  • CD molecules
  • Immunoglobulin like domain containing
  • Minor histocompatibility antigens
• VEGA ID
  OTTHUMG00000097049
• Locus Specific Databases
  • CD19base: Mutation registry for CD19 deficiency
  • LRG_35

Gene info

• Identity
  HGNC:1628
• Gene
  CD14
• Long gene name
  CD14 molecule
• Synonyms gene name
  • CD14 antigen
• Locus
  5q31.3
• Discovery year
  1988-05-31
• Entrez gene record
  929
• Pubmed identfication
  • 2472171
  • 2462937
• RefSeq identity
  • NM_000591
• Classification
  • CD molecules
  • Scavenger receptors
• VEGA ID
  OTTHUMG00000129507

Gene info

• Identity
  HGNC:34319
• Gene
  SNAR-C1
• Long gene name
  small NF90 (ILF3) associated RNA C1
• Synonyms gene name
  • small ILF3/NF90-associated RNA C1
• GenBank acession
  • EU071066
• Locus
  19q13.32
• Discovery year
  2008-07-03
• Entrez gene record
  100170225
• Pubmed identfication
  • 17855395
• RefSeq identity
  • NR_024220
• Classification
  • Small NF90 (ILF3) associated RNAs

Gene info

• Identity
  HGNC:12275
• Gene
  TRGC1
• Long gene name
  T cell receptor gamma constant 1
• Synonyms gene
  • TCRGC1
• Synonyms gene name
  • TCRGC1
• Synonyms
  • C1
• Synonyms name
  • T-cell receptor, gamma, constant region C1
• GenBank acession
  • M14996
• Locus
  7p14.1
• Discovery year
  1989-05-30
• Entrez gene record
  6966
• Pubmed identfication
  • 2879283
• RefSeq identity
  • NG_001336
• Classification
  • T cell receptor gamma locus at 7p14
• VEGA ID
  OTTHUMG00000155219

Gene info

• Identity
  HGNC:8868
• Gene
  PFDN4
• Long gene name
  prefoldin subunit 4
• Synonyms gene name
  • prefoldin 4
• Synonyms
  • PFD4
  • C-1
  • C1
• GenBank acession
  • U41816
• Locus
  20q13.2
• Discovery year
  1998-08-06
• Entrez gene record
  5203
• Pubmed identfication
  • 9630229
  • 8744932
• RefSeq identity
  • NM_002623
• Classification
  • Prefoldin subunits
• VEGA ID
  OTTHUMG00000032775

Gene info

• Identity
  HGNC:19737
• Gene
  TNS2
• Long gene name
  tensin 2
• Synonyms gene
  • TENC1
• Synonyms gene name
  • tensin like C1 domain-containing phosphatase
  • tensin like C1 domain containing phosphatase (tensin 2)
• Synonyms
  • KIAA1075
  • C1-TEN
• GenBank acession
  • AF518729
• Locus
  12q13.13
• Discovery year
  2002-12-09
• Entrez gene record
  23371
• RefSeq identity
  • NM_170754
• Classification
  • PTEN protein phosphatases
  • SH2 domain containing
  • C2 tensin-type domain containing
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000169730

Gene info

• Identity
  HGNC:674
• Gene
  ARHGAP4
• Long gene name
  Rho GTPase activating protein 4
• Synonyms
  • KIAA0131
  • C1
  • p115
  • RhoGAP4
  • SrGAP4
• Synonyms name
  • Rho-GAP hematopoietic protein C1
• GenBank acession
  • X78817
• Locus
  Xq28
• Discovery year
  1997-08-28
• Entrez gene record
  393
• Pubmed identfication
  • 8570618
• RefSeq identity
  • NM_001666
• Classification
  • AH/BAR family Rho GTPase activating proteins
  • F-BAR domain containing
• VEGA ID
  OTTHUMG00000024226
• Locus Specific Databases
  • Global Variome shared LOVD

Gene info

• Identity
  HGNC:6812
• Gene
  MAGEC1
• Long gene name
  MAGE family member C1
• Synonyms gene name
  • melanoma antigen family C1
• Synonyms
  • MAGE-C1
  • CT7
  • MGC39366
  • CT7.1
• Synonyms name
  • cancer/testis antigen family 7, member 1
• GenBank acession
  • AF064589
• Locus
  Xq27.2
• Discovery year
  1999-04-23
• Entrez gene record
  9947
• Pubmed identfication
  • 9485030
  • 9618514
• RefSeq identity
  • NM_005462
• Classification
  • MAGE family
• VEGA ID
  OTTHUMG00000022569
• Locus Specific Databases
  • Global Variome shared LOVD

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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