Rat Anti-Mouse CD86/B7-2-LE/AF
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Catalog numberGEN673458
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PricePlease ask
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Size500ug
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Also known asCD86/B7-2
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Other namesCD86/B7-2 costimulatory molecule; N/A; CD86/B7-2 costimulatory molecule; CD86 antigen; B7-1 costimulatory molecule; N/A; N/A
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CategorySecondary Antibodies
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SubcategoryMnoclonal antibodies
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Gene nameLOC554318; N/A; N/A; N/A
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Gene name synonimsLOC554318; N/A; N/A; N/A
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Other gene namesLOC554318; N/A; N/A; N/A
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ClonalityMonoclonal
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Immunoglobulin isotypeRat (Louvain) IgG2a kappa
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CloneGL1
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Host organismRat (Rattus norvegicus)
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Species reactivityMouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivityMouse CD86, the B7-2 co-stimulatory molecule and a ligand for CD28 and CD152/CTLA-4 CD86, also known as B7-2, is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes.^1-5 CD86 expression is rapidly upregulated by B-cell specific stimuli with peak expression at 18-42 hours after stimulation.^1, 5 CD86, along with CD80/B7-1, is an important accessory molecule in T cell costimulation via its interaction with CD28 and CD152/CTLA-4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response.^1-9 The monoclonal antibody GL1 blocks mixed lymphocyte reactions and stimulation of T cells by antigen-presenting cells.^1, 3, 5; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification methodN/A
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Form AppearanceLow Endotoxin, Azide - Free (LE/AF)
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ConcentrationN/A
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Storage and shippingThe purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or aminecontaining buffer salts added. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 7 (R-PE-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below Store the antibody at -20 degrees Celsius.. Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product the antibody should be stored at -20 degrees Celsius. immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
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Tested applicationsFlow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus, Rattus norvegicus
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target
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Gene symbolCD86, CD276, HHLA2, FUT3, RNY4P2, LRRC23, HPS4, PCDHGB7, NCR3LG1, CD274
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Short nameAnti-Mouse CD86/B7-2-LE/AF
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Techniqueanti-, Mouse, anti, antibody to, antibodies, mouses
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HostRat
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SpeciesMouse, Mouses, Rats
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Alternative nameAnti-Rat Anti-Mouse CD86/B7-2-LE/AF
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Alternative techniquemurine, antibodies
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Alternative to gene targetCD86 molecule, B7-2 and B7.2 and B70 and CD28LG2 and LAB72, CD86 and IDBG-52336 and ENSG00000114013 and 942, coreceptor activity, Cell surfaces, Cd86 and IDBG-158071 and ENSMUSG00000022901 and 12524, CD86 and IDBG-633264 and ENSBTAG00000013118 and 414345
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Gene info
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Identity
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Gene
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Long gene nameCD86 molecule
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Synonyms gene
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Synonyms gene name
- CD86 antigen (CD28 antigen ligand 2, B7-2 antigen)
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Synonyms
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Synonyms name
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Locus
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Discovery year1994-12-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- B7 family
- CD molecules
- V-set domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCD276 molecule
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Synonyms gene name
- CD276 antigen
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Synonyms
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GenBank acession
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Locus
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Discovery year2005-03-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C2-set domain containing
- V-set domain containing
- B7 family
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameHERV-H LTR-associating 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1999-09-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C1-set domain containing
- V-set domain containing
- B7 family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namefucosyltransferase 3 (Lewis blood group)
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Synonyms gene
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Synonyms gene name
- fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group included)
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Synonyms
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Synonyms name
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Locus
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Discovery year1993-04-08
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Fucosyltransferases
- Blood group antigens
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameRNY4 pseudogene 2
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Synonyms gene name
- RNA, Y4 small cytoplasmic (associated with Ro protein) pseudogene 2
- RNA, Ro-associated Y4 pseudogene 2
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Synonyms
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GenBank acession
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Locus
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Discovery year1993-08-25
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Entrez gene record
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RefSeq identity
Gene info
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Identity
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Gene
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Long gene nameleucine rich repeat containing 23
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Synonyms
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GenBank acession
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Locus
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Discovery year2006-01-24
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameHPS4 biogenesis of lysosomal organelles complex 3 subunit 2
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Synonyms gene name
- Hermansky-Pudlak syndrome 4
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Synonyms
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Locus
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Discovery year2001-06-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Biogenesis of lysosomal organelles complex 3
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameprotocadherin gamma subfamily B, 7
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-06-28
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Clustered protocadherins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenatural killer cell cytotoxicity receptor 3 ligand 1
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Synonyms
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Synonyms name
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Locus
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Discovery year2012-04-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Receptor ligands
- C1-set domain containing
- B7 family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCD274 molecule
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Synonyms gene
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Synonyms gene name
- programmed cell death 1 ligand 1
- CD274 antigen
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-11-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Receptor ligands
- C2-set domain containing
- V-set domain containing
- B7 family
- CD molecules
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VEGA ID