CD21, B-Cell (mature), Clone: WEHI-B2 (B21), Mouse Monoclonal antibody-Human, FITC
#
-
Catalog numberAMD09HLEUK20E
-
Price:
-
Size1000ul
-
-
Alternative name 1CD21, B-Cell (mature), Clone: WEHI-B2 (B21), Mab anti-Human, FITC
-
Alternative name 2CD21, B-Cell (mature), Clone: WEHI-B2 (B21), Mab antibody-Human, FITC
-
Alternative name 3CD21, B-Cell (mature), Clone: WEHI-B2 (B21), Mab antibody-Homo sapiens, FITC
-
Alternative name 4anti-CD21 B-Cell mature Clone: WEHI-B2 B21
-
Alternative name 5CD21 B-Cell mature Clone: WEHI-B2 B21
-
ClonalityMonoclonal Antibody
-
DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
-
Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
-
AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
-
PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. accurate-monoclonals FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Additional descriptionFor cells, cell lines and tissues in culture till half confluency.
-
ConjugationAnti-FITC Antibody
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin nameMus musculus
-
French translationanticorps
-
Gene target
-
Gene symbolCR2, SNAR-B2, PCDHGB2, IGKV5-2, ACAP2, SH3GL3, CYTH1, SIRPG, NABP1
-
Short nameCD21, B- (mature), Clone: WEHI-B2 (B21), Mouse Monoclonal antibody- , FITC
-
TechniqueMouse monoclonal, Antibody, Mouse, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
-
Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
-
LabelFITC
-
SpeciesHuman, Humans, Mouses
-
Alternative nameCD21, B-cellular (mature), clonality: WEHI-B2 (B21), Mouse monoclonal (antibody to-)-H. sapiens, fluorecein
-
Alternative techniqueantibodies, murine, fluorescine
-
Tissuecell
-
Gene info
-
Identity
-
Gene
-
Long gene namecomplement C3d receptor 2
-
Synonyms gene name
- complement component (3d/Epstein Barr virus) receptor 2
- complement component 3d receptor 2
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2001-06-22
-
Entrez gene record
-
RefSeq identity
-
Classification
- Sushi domain containing
- Complement system regulators and receptors
- CD molecules
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene namesmall NF90 (ILF3) associated RNA B2
-
Synonyms gene name
- small ILF3/NF90-associated RNA B2
-
GenBank acession
-
Locus
-
Discovery year2008-07-03
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Small NF90 (ILF3) associated RNAs
Gene info
-
Identity
-
Gene
-
Long gene nameprotocadherin gamma subfamily B, 2
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-06-28
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Clustered protocadherins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin kappa variable 5-2
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-04-18
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin kappa locus at 2p11.2
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameArfGAP with coiled-coil, ankyrin repeat and PH domains 2
-
Synonyms gene
-
Synonyms gene name
- centaurin, beta 2
-
Synonyms
-
Locus
-
Discovery year2001-09-03
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- BAR-PH domain containing
- Ankyrin repeat domain containing
- ArfGAPs
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameSH3 domain containing GRB2 like 3, endophilin A3
-
Synonyms gene name
- SH3 domain containing GRB2 like 3
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1996-10-26
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- N-BAR domain containing
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namecytohesin 1
-
Synonyms gene
-
Synonyms gene name
- pleckstrin homology, Sec7 and coiled-coil domains 1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1998-11-19
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Pleckstrin homology domain containing
- Cytohesins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namesignal regulatory protein gamma
-
Synonyms gene
-
Synonyms gene name
- signal-regulatory protein beta 2
- signal-regulatory protein gamma
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2001-09-17
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- CD molecules
- Ig-like cell adhesion molecule family
- C1-set domain containing
- Signal regulatory proteins
- V-set domain containing
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namenucleic acid binding protein 1
-
Synonyms gene
-
Synonyms gene name
- oligonucleotide/oligosaccharide-binding fold containing 2A
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2006-01-17
-
Entrez gene record
-
RefSeq identity
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data