Caspase-6, human recombinant
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Catalog number
1086-25
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Price
Please ask
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Size
25 units
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Synonyms
Caspase-6
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Alternative_names
Caspase-6, CASP-6, Apoptotic protease Mch-2
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Description
A cysteine-aspartic acid protease involved in apoptosis
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Recombinant
Yes
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Source
E. coli.
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Activity Specifications test method
> 13000 units/mg
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Unit Definition
One unit of the recombinant caspase-6 is the enzyme activity that cleaves 1 nmol of the caspase substrate VEID-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
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Molecular Weight
large (18 kDa) and small (11 kDa)
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Storage Temp
-70°C
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Shipping
gel pack
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Shelf Life
12 months
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Appearance
Lyophilized powder
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Physical form description
Lyophilized powder
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Reconstitution Instructions
Reconstitute to 1 unit per µl in PBS containing 15% glycerol.
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Background Information
Caspase-6 (also know as Mch2) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-6 also exists in cells as an inactive proenzyme. During apoptosis procaspase-6 is processed at aspartate residues by self-proteolysis and/or cleavage by another caspase. The processed active form of caspase-6 consists of large (18 kDa) and small (11 kDa) subunits which associate to form the active enzyme. Active caspase-6 has been shown involving in the proteolysis of PARP (poly ADP-ribose polymerase), an enzyme that is involved in DNA repair and henomic maintenance. The recombinant active human caspase-6 was expressed in E. coli. The active caspase-6 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-VEID-pNA (Cat. #1070-200) or Ac-VEID-AFC (1069-200).
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Additional description
Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
CASP6, CARD6
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Short name
Caspase-6, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Species
Human, Humans
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Alternative name
caspase-6, H. sapiens Rec.
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Alternative technique
rec
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Gene info
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Identity
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Gene
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Long gene name
caspase 6
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Synonyms gene name
- caspase 6, apoptosis-related cysteine protease
- caspase 6, apoptosis-related cysteine peptidase
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Synonyms
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GenBank acession
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Locus
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Discovery year
1996-07-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
MeSH Data
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Name
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Concept
Scope note:
Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
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Tree numbers
Product images
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