C1 Inhibitor, Human Recombinant
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Catalog number7120-50
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Price:
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Size50 ug
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SynonymsPlasma protease C1, inhibitor, C1inh, C1 Esterase Protein, C1-inhibiting factor, Serpin G1
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Alternative_namesPlasma protease C1, inhibitor, C1inh, C1 Esterase Protein, C1-inhibiting factor, Serpin G1
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DescriptionOne of the primary regulators of the immune complement system
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RecombinantYes
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SourceCHO cells
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Purity by SDS PAGE≥95%
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AssaySDS-PAGE
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Endotoxin Level< 0.1 ng/ug of protein (
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Activity Specifications test methodMeasured by its ability to inhibit recombinant human complement component C1a cleavage of a colorimetric peptide substrate, N Carbobenzyloxy-LysThioBenzyl ester (Z-K-SBzl). The expected IC50 is ≤ 2.6 nM
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Biological activityMeasured by its ability to inhibit recombinant human complement component C1a cleavage of a colorimetric peptide substrate, N Carbobenzyloxy-LysThioBenzyl ester (Z-K-SBzl). The expected IC50 is ≤ 2.6 nM
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Molecular Weight49.4 kDa
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized powder
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Physical form descriptionSterile filtered through a 0.2 micron filter. Lyophilized with 10 mM sodium phosphate, pH 7.5.
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Reconstitution InstructionsCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
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Background InformationC1 Inhibitor is a member of the serpin family of structurally related proteins, and is the primary regulator of the immune complement system. C1 Inhibitor is a protease inhibitor that functions to inhibit the complement system in order to prevent over-activation or spontaneous activation. Inhibition is achieved by binding to and irreversibly inhibiting the C1r and C1s proteases of the C1 complex, which has the effect of shutting down all subsequent downstream events in the complement activation cascade. C1inhibitor can also inhibit various other proteases, including Kallikrein, Factor XIa, and Factor XIIa. Deficiencies in C1inhibitor are the primary cause of hereditary angioedema (HAE, hereditary angioneurotic edema), a disease characterized by edema in the respiratory and gastrointestinal tracts. In certain clinical situations, the direct administration of C1inhibitor can be used to treat HAE and certain other conditions. Recombinant Human C1 Inhibitor is a highly glycosylated glycoprotein containing 445 amino acid residues (49.4kDa), corresponding to amino acids 56 – 500 of the C1 inhibitor precursor, and is fully functional in its ability to inhibit the C1 complex. Glycosylated C1 Inhibitor migrates at an apparent molecular weight of approximately 80-90 kDa by SDS PAGE analysis under reducing conditions.
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Amino acid sequenceVEPILEVSSL PTTNSTTNSA TKITANTTDE PTTQPTTEPT TQPTIQPTQP TTQLPTDSPT QPTTGSFCPG PVTLCSDLES HSTEAVLGDA LVDFSLKLYH AFSAMKKVET NMAFSPFSIA SLLTQVLLGA GENTKTNLES ILSYPKDFTC VHQALKGFTT KGVTSVSQIF HSPDLAIRDT FVNASRTLYS SSPRVLSNNS DANLELINTW VAKNTNNKIS RLLDSLPSDT RLVLLNAIYL SAKWKTTFDP KKTRMEPFHF KNSVIKVPMM NSKKYPVAHF IDQTLKAKVG QLQLSHNLSL VILVPQNLKH RLEDMEQALS PSVFKAIMEK LEMSKFQPTL LTLPRIKVTT SQDMLSIMEK LEFFDFSYDL NLCGLTEDPD LQVSAMQHQT VLELTETGVE AAAASAISVA RTLLVFEVQQ PFLFVLWDQQ HKFPVFMGRV YDPRA
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional descriptionTissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolSERPING1
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Short nameC1 Inhibitor, Recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameC1 suppressor, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nameserpin family G member 1
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Synonyms gene
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Synonyms gene name
- serine (or cysteine) proteinase inhibitor, clade G (C1 inhibitor), member 1, (angioedema, hereditary)
- serpin peptidase inhibitor, clade G (C1 inhibitor), member 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Complement system regulators and receptors
- Serpin peptidase inhibitors
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data