Bub1 Antibody
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Catalog numberbs-4294R
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PricePlease ask
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Size0.1ml
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Long nameBub1 Primary Polyclonal Antibody
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Also known asBub1 PAb
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CategoryPrimary Antibodies
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ConjugationUnconjugated
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Target AntigenBub1
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SpecificityThis is a highly specific antibody against Bub1.
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Modification sNone
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Modification site sUnmodified antibody
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ClonalityPolyclonal antibody
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Clone numberPolyclonal antibody
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Concentration1ug per 1ul
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Immunogen range980-1030/1085
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Subcellular locationNucleus
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SourceThis antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human Bub1
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Gene ID number699
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Swiss ProtO43683
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Tested ApplicationsWB, IHC-P, IF(IHC-P)
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Recommended dilutionsWB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
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Cross reactivityHuman, Mouse, Rat
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Cross reactive species detailsDue to limited amount of testing and knowledge, not every possible cross-reactivity is known.
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Background of the target antigenSerine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.
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Purification methodThis antibody was purified via Protein A.
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Storage conditionsKeep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
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Synonym namesBUB1A; BUB1L; hBUB1; Mitotic checkpoint serine/threonine-protein kinase BUB1; BUB1
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PropertiesIf you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolBUB1, BUB1P1, BUB1B
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Short nameAnti-Bub1
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TechniqueAntibody, antibodies against human proteins, antibodies for
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HostRabbit (Oryctolagus cuniculus)
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IsotypeImmunoglobulin G (IgG)
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Alternative nameBub1 Polyclonal Antibody
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Alternative techniqueantibodies
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Alternative to gene targetbudding uninhibited by benzimidazoles 1 homolog (yeast), BUB1A and BUB1L and hBUB1, BUB1 and IDBG-65670 and ENSG00000169679 and 699, transferase activity, nuclei, Bub1 and IDBG-205152 and ENSMUSG00000027379 and 12235, BUB1 and IDBG-628990 and ENSBTAG00000021181 and 514777
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Gene info
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Identity
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Gene
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Long gene nameBUB1 mitotic checkpoint serine/threonine kinase
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Synonyms gene
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Synonyms gene name
- budding uninhibited by benzimidazoles 1 (yeast homolog)
- budding uninhibited by benzimidazoles 1 homolog (yeast)
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-08-18
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameBUB1 mitotic checkpoint serine/threonine kinase pseudogene 1
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Locus
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Discovery year2014-01-08
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Entrez gene record
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameBUB1 mitotic checkpoint serine/threonine kinase B
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Synonyms gene name
- budding uninhibited by benzimidazoles 1 (yeast homolog), beta
- budding uninhibited by benzimidazoles 1 homolog beta (yeast)
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-03-25
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Entrez gene record
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Pubmed identfication
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data