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Available ordering format
Lyophilized
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Immunogen
Purified native protein from Pisum sativum
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Raised in
Guinea pig
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
For reconstitution add 100 µl of sterile water.Lyophilized antibody can be stored at -20 or -80°C. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB)
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Connected products
AS15 2880 | anti-ACCase subunit beta | acetyl-coenzyme A, carboxylase (subunit beta) rabbit antibodySecondary antibodies
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Recommended dilutions for use
1:2000 with ECL (WB)
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Molecular weight expected аpparent
29.6 kDa
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Verified reactivity
Arabidobsis thaliana, Hordeum vulgare, Pisum sativum, Solanum lycopersicum
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Possible reactivity
Hordeum vulgare, Nicotiana tabacum
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No reactivity
no confirmed exceptions from predicted reactivity are currently known
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Supplementary information
Higher protein load of 30-50 µg/well is needed for other species than Arabidopsis thaliana.
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References
to be added when available, antibody released in December 2015
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Scientific context
Biotin carboxyl carrier subunit of chloroplast ACCase is a biotin carboxyl-carrier subunit of the multi-enzyme plastidial acetyl-coenzyme A carboxylase complex involved in fatty acid biosynthesis process.
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Notes
None
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Protein number
F4KE21
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TAIR number
AT5G16390
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Description
A carrier can hold protected the subject it is supporting for the period needed by its application as stated by agisera.