-
Description
Bio Peptone, Certified is produced by a controlled enzymatic hydrolysis of casein and animal tissue. Used as a growth factor in culture media of large variety of microorganisms.
-
-
Appearance
Light yellow to brownish yellow Homogenous Free flowing powder ,having Characteristic odour but not putrescent
-
Solubility
Freely soluble in distilled/purified water, insoluble in alcohol and ether.
-
Clarity
1% w/v aqueous solution remains clear without haziness after autoclaving at 15 lbs pressure (121ºC) for 15 minutes
-
Reaction
Reaction of 2% w/v aqueous solution at 25ºC.
-
PH
6.00- 7.00
-
Total aerobic microbial count cfu gm
By plate method when incubated at 30-35°C for not less than 3 days. Bacterial Count :
-
Total Yeast and mould count cfu gm
By plate method when incubated at 20-25°C for not less than 5 days. Yeast & mould Count :
-
Test for Pathogens
1. E.coli-Negative in 10 gms of sample2. Salmonella species-Negative in 10 gms of sample3. Pseudomonas aeruginosaNegative in 10 gms of sample4. Staphylococcus aureus- Negative in 10 gms of sample5. C.albicans- Negative in 10 gms of sample6. Clostridia- Negative in 10 gms of sample
-
Cultural response
Cultural response observed after an incubation at 35-37ºC for 18-24 hours by preparing Columbia Broth (M145) using Biopeptone as an ingredient.
-
Chemical Analysis
TotalNitrogen >= 12.0% AminoNitrogen >= 2.50% Sodium chloride
-
Storage
Store below 30°C.
