Bilirubin (Total and Direct) Colorimetric Assay Kit

• Catalog number
  K553-100
• Price
  Please ask
• Size
  100 assays

• Description
  Allows measurement of both Total and Direct or Conjugated Bilirubin
• Summary
  • Detection method- Absorbance (600 nm for Total Bilirubin and 550 nm for Direct Bilirubin) • Application- Measurement of Bilirubin in Serum samples
• Detection Method
  Absorbance (600 nm for Total Bilirubin and 550 nm for Direct Bilirubin)
• Applications
  Measurement of Bilirubin in Serum samples
• Sample Type
  Biological fluids: Serum
• Features Benefits
  • Simple protocol, robust and reliable data • This assay allows detection of both Total and Direct or conjugated Bilirubin in serum samples.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Bilirubin, a degradation product of heme catabolism, is a non-polar molecule. There are two forms of bilirubin: water-soluble (conjugated or direct) and water-insoluble (unconjugated or indirect) bilirubin. Bilirubin is produced in the endoplasmic reticulum as unconjugated bilirubin, which binds to albumin in plasma and forms albumin-bilirubin complex. This complex is transported to the liver, where it is conjugated with glucuronic acid and forms conjugated bilirubin. Bilirubin has potent antioxidant, anti-inflammatory and autoimmune properties. Bilirubin concentration in human body depends on gender, drug intake, age, etc. Low serum bilirubin is directly correlated with pathological conditions including diabetes mellitus, metabolic syndrome, and cardiovascular diseases. However, high bilirubin indicates hemolysis, jaundice, Gilbert’s syndrome, hepatitis, drug toxicity, and possible blockage of bile ducts. BioVision’s Bilirubin Assay Kit utilizes the Jendrassik-Grof principle to detect bilirubin. Total bilirubin (unconjugated + conjugated) concentration is determined in the presence of a catalyst, where bilirubin reacts with a diazo- salt to form azobilirubin, which absorbs at 600 nm. Direct bilirubin (conjugated) is determined in the absence of catalyst (550 nm). .
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Bilirubin   Direct   Kit  
• Short name
  Bilirubin ( Direct) Colorimetric Assay Kit
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Bilirubin (Total and Direct) colorimetry test reagent
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  total

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