beta Glucuronidase Antibody
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Catalog number
R31016
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Price
Please ask
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Size
0.1mg
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Category
Antibody
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Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
beta Glucuronidase
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB
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Recommended dilutions
Western blot: 0.5-1ug/ml
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Notes
The stated application concentrations are suggested starting amounts. Titration of the beta Glucuronidase antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This beta Glucuronidase antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
P08236
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Purity
Antigen affinity
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Description
Beta-Glucuronidase is an enzyme that in humans is encoded by the GUSB gene, a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans. It is a tetrameric glycoprotein composed of identical subunits. The GUSB gene is mutated in mucopolysaccharidosis type VII. Miller et al.(1990) reported that the gene is 21 kb long and contains 12 exons. T-Beta-glucuronic acid acts naturally to cleave glucuronides from conjugated bilirubin to produce unconjugated bilirubin, which is then absorbed into the bloodstream.Kreamer et al.(2001) postulated that the beta-Glucuronidase inhibitor L-aspartic acid can thus block enterohepatic circulation of bilirubin. Tomatsu et al.(2002) stated that more than 45 different mutations in the GUSB gene had been identified in human patients with MPS VII, approximately 90% of which were point mutations.
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Immunogen
An amino acid sequence from the N-terminus of human GUSB (DFSDNRRRGFEEQWYRRPLWE) was used as the immunogen for this beta Glucuronidase antibody.
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Storage
After reconstitution, the beta Glucuronidase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
GUSB, GUSBP8
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Short name
Anti-beta Glucuronidase
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit IgG
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Alternative name
Antibodies to beta Glucuronidase
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Alternative technique
antibodies
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Gene info
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Identity
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Gene
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Long gene name
glucuronidase beta
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Synonyms gene name
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GenBank acession
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Locus
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Discovery year
2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
GUSB pseudogene 8
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Synonyms gene name
- glucuronidase, beta pseudogene 8
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Locus
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Discovery year
2011-06-09
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Entrez gene record
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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