Beclin 1 Antibody
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Catalog numberF46280-0.08ML
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PricePlease ask
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Size0.08 ml
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CategoryAntibody
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ConcentrationIn 1X PBS pH 7.4 with 0.09% sodium azide
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FormPurified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenBeclin 1
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens), Mouse (Mus musculus) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsFACS, WB, IHC, ELISA
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Recommended dilutionsWestern blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
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NotesTitration of the Beclin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis Beclin antibodyis to be used only for research purposes and not for diagnostics..
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UniprotQ14457
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PurityPurified
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DescriptionMacroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Beclin 1 plays a role in two fundamentally important cell biological pathways: autophagy and apoptosis. Beclin 1 is thought to function as a VPS and autophagy protein as part of a complex with Class III PI3 kinase, Vps34.
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ImmunogenA portion of amino acids 210-239 from the human protein was used as the immunogen for this Beclin antibody.
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StorageAliquot the Beclin antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolBECN2, BECN1, BECN1P2
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Short nameAnti-Beclin 1
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit Ig
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Alternative nameAntibodies to Beclin 1
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namebeclin 2
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Synonyms gene
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Synonyms gene name
- beclin 1, autophagy related, pseudogene 1
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Locus
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Discovery year2010-06-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namebeclin 1
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Synonyms gene name
- beclin 1 (coiled-coil, moesin-like BCL2 interacting protein)
- beclin 1, autophagy related
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-11-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- PIK3C3 complex subunits
- MicroRNA protein coding host genes
- Autophagy related
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namebeclin 1 pseudogene 2
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Synonyms gene name
- beclin 1, autophagy related, pseudogene 2
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Locus
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Discovery year2014-10-03
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Entrez gene record
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data