BCA Protein Assay Kit - Reducing Agent Compatible
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Catalog numberK818-1000
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Price:
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Size1000 assays
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DescriptionOnly kit on the market that is compatible with higher conc. of reducing agents TCEP (20 mM), DTT (10 mM), β-ME (35 mM)
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Summary• Detection method- Absorbance (562 nm) • Reducing agent compatible
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Detection MethodAbsorbance (562 nm)
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ApplicationsMeasuring total protein concentration of pure proteins, extracts or lysates in the presence of reducing agents.
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Sample Typepure proteins, cell/tissue extracts or lysates
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Features Benefits• Simple & convenient. • Optimized for determining total protein content of samples containing strong reducing agents such as DTT (10 mM), TCEP (20 mM) & β-mercaptoethanol (35 mM). • The assay is linear over a wide range of protein concentration (25-2000 μg/ml).
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Storage ConditionsRT
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Shipping ConditionsRT
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Shelf life12 months
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BackgroundBioVision’s Reducing Agent Compatible BCA Protein Assay Kit is the only protein estimation kit on the market that is compatible with strong reducing agents such as TCEP up to 20 mM, DTT up to 10 mM & β-mercaptoethanol up to 35 mM. This kit is adapted from BioVision’s BCA Assay Kit II and is based on the chelation of bicinchoninic acid (BCA) with the cuprous cation (Cu+1), which is generated by reduction of cupric cation (Cu+2) with the protein in alkaline conditions. Reducing agents in samples interfere with BCA assay due to their ability to reduce Cu+2. BioVision’s Reducing Agent Compatible BCA Protein Assay Kit is useful for determing protein concentrations of samples containing DTT, TCEP & β-mercaptoethanol. The assay is linear over a wide range of protein concentration between 25-2000 μg/ml. In general, protein concentrations are calculated with reference to a commonly used protein standard. The kit also includes Bovine Serum Albumin (BSA) as a protein standard for estimation of total protein content of samples.
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TestBiovision supplies other types of Assays as 1.
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Gene target
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Gene symbolCXCL13, BLNK
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Short nameBCA Protein Assay Kit - Reducing Agent Compatible
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TechniqueAssay, assays
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HostAssay
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Alternative nameBicinchoninic acid Protein test reagent - Reducing substance Compatible
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Alternative techniquearrays, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
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Identity
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Gene
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Long gene nameC-X-C motif chemokine ligand 13
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Synonyms gene
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Synonyms gene name
- small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant)
- chemokine (C-X-C motif) ligand 13
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-09-05
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Entrez gene record
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Pubmed identfication
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Classification
- Chemokine ligands
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameB cell linker
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-07-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- SH2 domain containing
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VEGA ID
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Locus Specific Databases