BARD1 Antibody
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Catalog number3768-100
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Price:
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Size100 µl
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Antibody TargetBARD1
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Target Alternative NameBARD1, BARD-1
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Antibody TypePolyclonal
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Species ReactivitiesHuman
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Immunogen SequenceSynthetic peptide of human BARD1
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AccessionQ99728
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Gene Id580
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AppearanceColorless liquid
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FormLiquid
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Formulation100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.
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PurificationAffinity purified
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ApplicationWestern blot, Immunoprecipitation
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Application And UsagesWestern blotting (1:500 – 1:2500) and Immunoprecipitation. HeLa nuclear extract (Cat.# 1641-1) can be used as a positive control. However, the optimal concentrations should be determined individually. The antibody recognizes the BARD1 of human origin. Reactivity to other species has not been tested.
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DescriptionMutations within the BRCA1 gene, localized to chromosome 17q, are believed to account for approximately 45% of families with increased incidence of both early-onset breast cancer and ovarian cancer. The BRCA1 gene is expressed in numerous tissues, including breast and ovary, and encodes a predicted protein of 1863 amino acids. This protein contains a RING domain near the N-terminus and appears to encode a tumor suppressor. BARD1 (BRCA1-associated RING domain protein 1) and BAP1 (BRCA1-associated protein 1) have both been shown to bind to the N-terminus of BRCA1 and are potential mediators of tumor suppression. BARD1 contains an N-terminal RING domain and three tandem ankyrin repeats. The C-terminus of BARD1 contains a region with sequence homology to BRCA1, termed the BRCT domain. BAP1 is a ubiquitin hydrolase and has been shown to enhance BRCA1-mediated cell growth suppression.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolBARD1
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Short nameBARD1 Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative namebreast cancer 1, early onset associated RING domain 1 (antibody to-)
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Alternative techniqueantibodies
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Alternative to gene targetBRCA1 associated RING domain 1, BARD1 and IDBG-80113 and ENSG00000138376 and 580, protein heterodimerization activity, nuclei, Bard1 and IDBG-164443 and ENSMUSG00000026196 and 12021, BARD1 and IDBG-643630 and ENSBTAG00000033690 and 530961
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Gene info
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Identity
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Gene
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Long gene nameBRCA1 associated RING domain 1
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Locus
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Discovery year1998-08-05
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ankyrin repeat domain containing
- Ring finger proteins
- BRCA1 B complex
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data