B-RAF (Phospho-Thr599) Antibody

Applications

IHC ELISA
Source

Rabbit
Reactivity

H, M, R
MW kDa

84 kDa
Target Modification

Phospho
Modification Sites

H:T599 M:T636 R:T636
Immunogen

The antiserum was produced against synthesized peptide derived from human B-RAF around the phosphorylation site of Thr599.
Immunogen Range

571-620
Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Specificity

B-RAF (Phospho-Thr599) Antibody detects endogenous levels of B-RAF only when phosphorylated at Thr599.
Gene ID

673
Swiss Prot No

P15056
OMIM No

114500/115150/164757/211980/605027
Unigene No

Hs.550061
NCBI Gene Symbol

BRAF1
Synonyms

B-RAF; B-RAF proto-oncogene serine/threonine-protein kinase; B-Raf proto-oncogene serine/threonine-protein kinase; BRAF; BRAF1; c-RMIL; kinase B-Raf; p94; RAFB1; RMIL; RMIL serine/threonine-protein kinase; v-Raf murine sarcoma viral oncogene homolog B1
Storage Stability

Stable at -20°C for at least 1 year.
Physical Form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality

Polyclonal
Concentration

1 mg/ml
Properties

If you buy Antibodies supplied by Assay Biotech they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation

anticorps

Identity

HGNC:11153
Gene

SNRPB
Long gene name

small nuclear ribonucleoprotein polypeptides B and B1
Synonyms gene
- SNRPB1
Synonyms
- COD
- SmB/SmB'
- Sm-B/B'
- snRNP-B
Locus

20p13
Discovery year

1988-11-28
Entrez gene record

6628
Pubmed identfication
- 1376292
Classification
- Small nucleolar RNA protein coding host genes
- Sm spliceosomal proteins
VEGA ID

OTTHUMG00000031694

Identity

HGNC:3424
Gene

ERAL1
Long gene name

Era like 12S mitochondrial rRNA chaperone 1
Synonyms gene name
- Era (E. coli G-protein homolog)-like 1
- Era G-protein-like 1 (E. coli)
- Era-like 12S mitochondrial rRNA chaperone 1
Synonyms
- HERA-B
GenBank acession
- AF082657
Locus

17q11.2
Discovery year

1999-11-19
Entrez gene record

26284
Pubmed identfication
- 10945472
- 20604745
VEGA ID

OTTHUMG00000132675

Identity

HGNC:8181
Gene

OR1B1
Long gene name

olfactory receptor family 1 subfamily B member 1
Synonyms gene name
- olfactory receptor, family 1, subfamily B, member 1 (gene/pseudogene)
- olfactory receptor family 1 subfamily B member 1 (gene/pseudogene)
Synonyms
- OR9-B
GenBank acession
- AC006313
Locus

9q33.2
Discovery year

1999-12-09
Entrez gene record

347169
RefSeq identity
- NM_001004450
Classification
- Olfactory receptors, family 1
VEGA ID

OTTHUMG00000020616

Identity

HGNC:4932
Gene

HLA-B
Long gene name

major histocompatibility complex, class I, B
Synonyms gene
- AS
Synonyms gene name
- ankylosing spondylitis
GenBank acession
- M15470
Locus

6p21.33
Discovery year

2001-06-22
Entrez gene record

3106
Pubmed identfication
- 3459708
RefSeq identity
- NM_005514
Classification
- C1-set domain containing
- Histocompatibility complex
- MicroRNA protein coding host genes
VEGA ID

OTTHUMG00000031153
Locus Specific Databases
- IMGT/HLA Database

Identity

HGNC:9956
Gene

RELB
Long gene name

RELB proto-oncogene, NF-kB subunit
Synonyms gene name
- v-rel avian reticuloendotheliosis viral oncogene homolog B (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3)
Synonyms
- REL-B
GenBank acession
- M83221
Locus

19q13.32
Discovery year

1995-10-02
Entrez gene record

5971
Pubmed identfication
- 1531086
Classification
- IPT domain containing
- NF-kappa B complex subunits
VEGA ID

OTTHUMG00000162116

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data