Aurora-A Antibody (AURKA)
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Catalog number
F50015-0.08ML
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Price
Please ask
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Size
0.08 ml
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Category
Antibody
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Concentration
In 1X PBS, pH 7.4, with 0.09% sodium azide
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Form
Purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
Aurora-A (AURKA)
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB, IF, FACS, ELISA
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Recommended dilutions
Western blot: 1:1000,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50
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Notes
Titration of the Aurora-A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This Aurora-A antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
O14965
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Purity
Purified
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Description
Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases, members of the Ser/Thr protein kinase family, associate with microtubules during chromosome movement and segregation. Auroria kinase A may play a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. It may be involved in microtubule formation and/or stabilization. This protein has also been postulated to play a key role during tumor development and progression. Aurora kinase A localizes on centrosomes in interphase cells and at each spindle pole in mitosis. It is highly expressed in testis, weakly in skeletal muscle, thymus and spleen, and also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines. Expression is cell-cycle regulated, low in G1/S, accumulates during G2/M, and decreases rapidly afterward. Defects in Aurora kinase A are responsible for numerical centrosome aberrations including aneuploidy.
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Immunogen
A portion of amino acids 364-392 from the human protein was used as the immunogen for this Aurora-A antibody.
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Storage
Aliquot the Aurora-A antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
AURKA
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Short name
Anti-Aurora-A (AURKA)
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit Ig
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Alternative name
Antibodies to Aurora-A (AURKA)
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Alternative technique
antibodies
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Gene info
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Identity
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Gene
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Long gene name
aurora kinase A
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Synonyms gene
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Synonyms gene name
- serine/threonine kinase 15
- serine/threonine kinase 6
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1998-10-02
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Protein phosphatase 1 regulatory subunits
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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