ATG7 Antibody
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Catalog numberR31385
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PricePlease ask
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Size0.1mg
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CategoryAntibody
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Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
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FormAntigen affinity purified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenATG7
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB
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Recommended dilutionsWestern blot: 0.5-1ug/ml
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NotesTitration of the ATG7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis ATG7 antibodyis to be used only for research purposes and not for diagnostics..
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UniprotO95352
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PurityAntigen affinity
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DescriptionAutophagy-related protein 7 is a protein that in humans is encoded by the ATG7 gene. This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein also shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. ATG7 is essential for the Apg12 conjugation system that mediates membrane fusion in autophagy. It is found that when nutrients are limited, the protein can regulate p53-dependent cell cycle and cell death pathways.
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ImmunogenAmino acids 652-669 (KVLDQYEREGFNFLAKVF-human) were used as the immunogen for this ATG7 antibody.
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StorageAfter reconstitution, the ATG7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolATG7
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Short nameAnti-ATG7
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameAntibodies to ATG7
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameautophagy related 7
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Synonyms gene
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Synonyms gene name
- APG7 autophagy 7-like (S. cerevisiae)
- ATG7 autophagy related 7 homolog (S. cerevisiae)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2003-11-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ubiquitin like modifier activating enzymes
- Autophagy related
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data