-
Gene info
SCRAPPER Antibody: Members of the F-box protein family, such as Scrapper, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases. Scrapper is selectively expressed in the brain, broadly expressed within the mouse CNS and is abundant at presynaptic membrane. Scrapper has orthologs in C. elegans, D. melanogaster, and mammals which suggests that it might function as an important membrane-localized E3 ligase in various species. Scrapper is a major presynaptic E3 ubiquitin ligase that acts through RIM1a via degradation and the ubiquitin-proteasome-system (UPS) pathway to critically regulate synaptic transmission. This identifies protein degradation as a mechanism for holding synaptic communication in check.
-
Gene names
SCRAPPER Antibody: Fbl2, Fbl20, FBL2, F-box and leucine-rich repeat protein 20
-
Antibodie s label
unlabelled serum
-
Purity
SCRAPPER Antibody is affinity chromatography purified via peptide column.
-
Ab raised in
Rabbit
-
Type
Polyclonal
-
Immunoglobulin
IgG
-
Antigen
SCRAPPER antibody was made against a 18 amino acid synthetic peptide near the amino terminus of the human SCRAPPER.
-
Immuno application
ELISA, Western Blot, immuno-histo-chemistry, immuno fluorescence
-
Antibody advice
SCRAPPER antibody can be used for detection of SCRAPPER by Western blot at 0.5 - 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL.
-
Suspention pH azide
SCRAPPER Antibody is supplied in PBS containing 0.02% sodium azide.
-
Keep at
SCRAPPER antibody can be stored at 4 grades C for three months and -20 grades C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
-
Works with
Human, Mouse, Rat
-
Omim nr
27734755
-
Ncbi nr
NP_116264
-
Vial with antibody
in solution
-
Quantity per ml
1 mg/mL
-
NCBI GENE nr
84961
-
GENE symbol
FBXL20
-
Antigene name
F-box and leucine-rich repeat protein 20
-
Source
Homo sapiens
-
Protein nr
Q96IG2
-
-
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translation
anticorps
