This antibody has been succesfully tested for use in following methods: Western blot, Immunoprecipitation, Immunohistochemistry
Target of this antibody: Phospho-STAT3
Formulation of this antibody: 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.
Alternative names of antibody target: MADH3 , JV15-2 , DKFZp686J10186 , hSMAD3 , hMAD-3 , HSPC193 , MGC60396 , HsT17436 , Mad3 , DKFZP586N0721
Recommended usage: Western blotting (1-4 µg/ml), immunoprecipitation (20 mg/ml), and Immunohistochemistry (20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~86 kDa phosphorylated Stat3 (Ser727) of human and mouse origins. Reactivity to other species has not been tested.
Storage information: Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions: gel pack
Antibody host: Rabbit
This antibody reacts with: Human, mouse
Clonality of antibody: Polyclonal
Isotype: Rabbit IgG
Purification method: Affinity purified
Immunogene sequence: Synthetic phospho-peptide corresponding to resides surrounding Ser727 of mouse Stat3
How to handle this antibody: The antibody solution should be gently mixed before use.
Background information: Membrane receptor signaling by various ligands induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Althoμgh early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 is activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.
Form of antibody: Liquid
Concentration of antibody: 0.5 mg/ml
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation anticorps
Short name anti-Phospho-Stat3 Antibody
Technique Antibody, anti
Alternative name antibody to-phosphorilated-signal transducer and activator on transcription 3 (acute-phase response factor) (Antibody to)
Alternative technique antibodies
Alternative to gene target signal transducer and activator of transcription 3 (acute-phase response factor), ADMIO and APRF and HIES, STAT3 and IDBG-50702 and ENSG00000168610 and 6774, protein dimerization activity, nuclei, Stat3 and IDBG-211637 and ENSMUSG00000004040 and 20848, STAT3 and IDBG-640197 and ENSBTAG00000021523 and 101910056,508541