Phospho-Lck Antibody
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Catalog number3499-100
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PricePlease ask
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Size100 µg
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Antibody TargetPhospho-Lck
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Target Alternative NameLCK , p56-LCK , p56lck , YT16 , pp58lck , LSK
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Antibody TypePolyclonal
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Species ReactivitiesHuman
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Immunogen SequenceSynthetic peptide corresponding to amino acids around Tyr505 of human Lck
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AccessionP06239
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Gene Id3932
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AppearanceColorless liquid
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FormLiquid
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Concentration0.5 mg/ml
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Formulation100 µg (0.5 mg/ml) antigen affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.
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PurificationAffinity purified
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ApplicationImmunoprecipitation, Immunohistochemistry
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Application And UsagesWestern blotting (1-2 µg/ml), immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (20-25 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 56 kDa phosphorylated Lck (Tyr505) of human, mouse and rat origins. Reactivity to other species has not been tested.
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DescriptionLck is a membrane associated tyrosine protein kinases. The Lck gene has been shown to undergo rearrangement and overexpression in some murine lymphomas. In human studies, it has been demonstrated that the Lck gene is localized to a site in the genome which undergoes frequent chromosomal abnormalities in lymphomas and neuroblastomas. Resting T cells contain high levels of the Lck protein and mRNA both of which decline upon activation of the T cells. These and other observations sμggest that alterations in Lck expression may contribute to the pathogenesis of some types of neoplastic disease.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Short namePhospho-Lck Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative namephosphorilated-lymphocyte-specific protein tyrosine kinase (antibody to-)
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Alternative techniqueantibodies
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Alternative to gene targetlymphocyte-specific protein tyrosine kinase, IMD22 and LSK and p56lck and pp58lck and YT16, LCK and IDBG-95667 and ENSG00000182866 and 3932, transferase activity, Cell surfaces, Lck and IDBG-191117 and ENSMUSG00000000409 and 16818, LCK and IDBG-644592 and ENSBTAG00000012695 and 508890
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data