anti-APRIL Antibody [Sacha-1]

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  • Catalog number
    XA-1014
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  • Size
    0.1 mg
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  • Gene names
    APRIL, TALL2, ZTNF2, UNQ383/PRO715, Tumor necrosis factor ligand superfamily member 13, A proliferation-inducing ligand, APRIL, CD256, TALL-2, TRDL-1
  • Antibodie s label
    unlabelled serum
  • Purity
    APRIL (monoclonal Sache-1) antibody was purified to ≥95% in SDS-PAGE.
  • Ab raised in
    Rat
  • Type
    Monoclonal
  • Monoclonal
    Sacha-1
  • Immunoglobulin
    IgG2a
  • Antigen
    APRIL (monoclonal Sache-1) antibody was made against recombinant human APRIL (aa. 105-250).
  • Immuno application
    immuno-cyto-chemistry, FLOW
  • Antibody advice
    APRIL antibody can be used to recognize human and mouse APRIL by Flow Cytometry (excellent for mouse APRIL, also stains human APRIL), and Immunocytochemistry. Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone. Slides were blocked with IgG, and incubated for 1 hour with 5 ug/mL Sacha-1 or control murine IgG (isotype control) in 1%BSA / 1x PBS for 1 hour. After washes wish PBS, samples were incubated with the secondary Ab for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC. Method: 3 HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mAPRIL (thick line). Cells were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1 ug/mL of Sacha-1 antibody. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.
  • Suspention pH azide
    PBS containing 0.02% sodium azide
  • Keep at
    APRIL antibody can be stored at 4 grades C, stable for one year.
  • Omim nr
    13124605
  • Ncbi nr
    O75888
  • Vial with antibody
    in solution
  • NCBI GENE nr
    8741
  • GENE symbol
    TNFSF13
  • Antigene name
    tumor necrosis factor (ligand) superfamily, member 13
  • Source
    Homo sapiens
  • Protein nr
    O75888
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Properties
    If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
  • French translation
    anticorps
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  • Gene target
  • Gene symbol
    TNFSF13, ANP32B
  • Short name
    anti-APRIL Antibody [Sacha-1]
  • Technique
    Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
  • Alternative name
    Antibody toAPRIL (antibody to-) [Sacha-1]
  • Alternative technique
    antibodies
Gene info
  • Identity
  • Gene
  • Long gene name
    TNF superfamily member 13
  • Synonyms gene name
    • tumor necrosis factor (ligand) superfamily, member 13
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1998-12-04
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Tumor necrosis factor superfamily
    • CD molecules
  • VEGA ID
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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