anti-APRIL Antibody [Sacha-1]
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Catalog numberXA-1014
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Price:
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Size0.1 mg
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Gene namesAPRIL, TALL2, ZTNF2, UNQ383/PRO715, Tumor necrosis factor ligand superfamily member 13, A proliferation-inducing ligand, APRIL, CD256, TALL-2, TRDL-1
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Antibodie s labelunlabelled serum
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PurityAPRIL (monoclonal Sache-1) antibody was purified to ≥95% in SDS-PAGE.
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Ab raised inRat
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TypeMonoclonal
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MonoclonalSacha-1
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ImmunoglobulinIgG2a
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AntigenAPRIL (monoclonal Sache-1) antibody was made against recombinant human APRIL (aa. 105-250).
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Immuno applicationimmuno-cyto-chemistry, FLOW
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Antibody adviceAPRIL antibody can be used to recognize human and mouse APRIL by Flow Cytometry (excellent for mouse APRIL, also stains human APRIL), and Immunocytochemistry. Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with acetone. Slides were blocked with IgG, and incubated for 1 hour with 5 ug/mL Sacha-1 or control murine IgG (isotype control) in 1%BSA / 1x PBS for 1 hour. After washes wish PBS, samples were incubated with the secondary Ab for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC. Method: 3 HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mAPRIL (thick line). Cells were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1 ug/mL of Sacha-1 antibody. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.
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Suspention pH azidePBS containing 0.02% sodium azide
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Keep atAPRIL antibody can be stored at 4 grades C, stable for one year.
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Omim nr13124605
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Ncbi nrO75888
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Vial with antibodyin solution
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NCBI GENE nr8741
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GENE symbolTNFSF13
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Antigene nametumor necrosis factor (ligand) superfamily, member 13
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SourceHomo sapiens
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Protein nrO75888
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesIf you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolTNFSF13, ANP32B
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Short nameanti-APRIL Antibody [Sacha-1]
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TechniqueAntibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative nameAntibody toAPRIL (antibody to-) [Sacha-1]
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameTNF superfamily member 13
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Synonyms gene name
- tumor necrosis factor (ligand) superfamily, member 13
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-12-04
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Tumor necrosis factor superfamily
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameacidic nuclear phosphoprotein 32 family member B
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Synonyms gene name
- acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2002-02-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- ANP32 acidic nuclear phosphoproteins
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data