MeCP2 polyclonal antibody
-
Catalog number
6827-25
-
Price
Please ask
-
Size
25 μg
-
-
Antibody Target
MeCP2
-
Target Alternative Name
AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RTS, RTT
-
Antibody Type
Polyclonal
-
Species Reactivities
Human.
-
Immunogen Sequence
MeCP2 (Methyl-CpG-binding domain protein 2) synthetic peptide containing a sequence from the C-terminal conjugated to KLH
-
Accession
P51608
-
Gene Id
4204
-
Appearance
Colorless liquid
-
Form
Liquid
-
Formulation
In PBS with 0.05% sodium azide and 0.05% ProClin 300.
-
Purification
Affinity Purified
-
Application
Western blot, ELISA, ChIP.
-
Positive Control
Western blot: Hela cells, ELISA: peptides, ChIP: U2OS cells.
-
Application And Usages
ChIP: 5 µg/ChIP, WB: 1:1000, ELISA: 1:1000
-
Description
MeCP2 is a chromosomal protein with abundant binding sites in the chromatin. It belongs to the family of methyl CpG binding proteins which also comprises MBD1, MBD2, MBD3 and MBD4. MeCP2 can bind specifically to methylated promoters, thereby repressing transcription. This transcriptional repression is mediated through interaction with histone deacetylase and the corepressor SIN3A. MeCP2 also is essential for development. Mutations in MeCP2 are the cause of several types of mental retardation including Rett syndrome, a progressive neurological disorder that causes mental retardation in females and mental retardation syndromic X-linked type 13, and may also be involved in Angelman syndrome and susceptibility to some types of autism.
-
Storage Condition
-20 °C
-
Shipping Condition
gel pack
-
Shelf Life
12 months
-
Usage
For Research Use Only! Not to be used in humans.
-
Handling
The antibody solution should be gently mixed before use.
-
Western Blot Verified
Verified
-
Immunocytochemistry Verified
Not Verified
-
Immunofluorescence Verified
Not Verified
-
Immunoprecipitation Verified
Not Verified
-
ELISA Verified
Verified
-
ChIP Verified
Verified
-
Dot Blot Verified
Not Verified
-
Flow Cytometry Verified
Not Verified
-
-
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
Group
Polyclonals and antibodies
-
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
-
French translation
anticorps
-
Gene target
-
Gene symbol
MECP2
-
Short name
MeCP2 polyclonal antibody
-
Technique
Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
-
Isotype
Rabbit IgG
-
Alternative name
MeCP2 polyclonal (antibody to-)
-
Alternative technique
polyclonals, antibodies
-
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Similar products