QuickDetect™ 5-lipoxygenase (5-LOX) (Human) ELISA Kit
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Catalog number
E4704-100
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Price
Please ask
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Size
96 assays
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Description
A Sandwich ELISA kit for the quantitative measurement of 5-lipoxygenase in human serum, plasma, culture media samples
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Detection Method
Absorbance-450 nm
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Species Reactivity
Human
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Applications
Detection range: 1U/L - 480 U/L, Assay Precision: Intra-Assay: CV
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Sample Type
Plasma, Cell and tissue culture supernatants, Serum, Other biological fluids
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Features Benefits
Detection of 5-lipoxygenase in human serum, plasma, culture media samples., Assay range 1U/L - 480 U/L, Sensitivity： 1U/L
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Storage Conditions
4°C
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Shipping Conditions
Gel Pack
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Shelf life
12 months
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Background
BioVision's 5-lipoxygenase ELISA kit is based on Sandwich ELISA method. The Microelisa stripplate provided in this kit has been pre-coated with an antibody specific to 5-LOX. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for 5-LOX is added to each Microelisa stripplate well and after incubation, all unbound components are washed away. The TMB substrate solution is added to each well. The wells that contain 5-LOX and HRP conjugated 5-LOX antibody will appear blue in color and turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of 5-LOX. The concentration of 5-LOX in the samples can be calculated by comparing the OD of the samples to the standard curve.
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Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
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Gene target
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Gene symbol
ALOX5, LOX, ALOX12B, ALOX12, ALOX15, ALOX15B, ALOXE3, OLR1, MIR1302-5, PIRC23
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Short name
QuickDetect™ 5-lipoxygenase (5-LOX) ( ) ELISA Kit
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Technique
ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
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Host
antigen assay ELISA kit 1 Kit (96 Wells)
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Species
Humans
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Alternative name
QuickDetect™ 5-lipoxygenase (5-LOX) (H. sapiens) Enzyme-linked immunosorbent assay reagent
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Alternative technique
elisas, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Gene info
Gene info
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Identity
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Gene
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Long gene name
lysyl oxidase
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Synonyms name
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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Pubmed identfication
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VEGA ID
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Locus Specific Databases
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
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Identity
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Gene
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Long gene name
microRNA 1302-5
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Synonyms gene
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Synonyms
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Locus
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Discovery year
2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
Gene info
MeSH Data
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Name
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Concept
Scope note:
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
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Tree numbers
- E05.478.566.350.170
- E05.478.566.380.360
- E05.478.583.400.170
- E05.601.470.350.170
- E05.601.470.380.360
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Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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