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Target antigen
Lipoprotein lipase
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Clonality
Polyclonal antibody
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Clone
Polyclonal antibody
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Raised in
rabbit
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Type of the antibody
IgG polyclonal antibody
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Product form
freeze-dried
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Reacts with species
human, rat Predicted to work with: mouse
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Analyses
WB
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Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Lipoprotein lipase(448-170aa EKVSHLQKGKAPAVFVKCHDKSL), different from the related mouse and rat sequences by two amino acids.
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Product configuration
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
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Purification
Immunogen affinity purified.
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Solubilization
The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml
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Storage condtions
Keep the Lipoprotein lipase Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The Lipoprotein lipase Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
Lipoprotein lipase(LPL) is the central enzyme in plasma triglyceride hydrolysis and is secreted by macrophages in the subendothelial space. Evidence has been provided that LPL produced by macrophages in the vessel wall exerts proatherogenic effects. Lipoprotein lipase has been difficult to purify, and its protein sequence remained undetermined until it could be deduced from the nucleotide sequence of its cDNA. The gene encodes a protein of 475 amino acids that becomes a mature protein of 448 residues after cleavage of a signal peptide. Analysis of the sequence indicated that human lipoprotein lipase, hepatic lipase, and pancreatic lipase are members of a gene family. The atherogenic effects of LPL have been mainly attributed to its ability to favor lipid accumulation within macrophages present in the atherosclerotic lesion.
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Related articles
1. Wion, K. L.; Kirchgessner, T. G.; Lusis, A. J.; Schotz, M. C.; Lawn, R. M. : Human lipoprotein lipase complementary DNA sequence. Science 235: 1638-1641, 1987. 2. Gotoda T., Senda M., Gamou T., Furuichi Y., Oka K.;Nucleotide sequence of human cDNA coding for a lipoprotein lipase (LPL) cloned from placental cDNA library."; Nucleic Acids Res. 17:2351-2352(1989). 3. Lo, J. Y.; Smith, L. C.; Chan, L. : Lipoprotein lipase: role of intramolecular disulfide bonds in enzyme catalysis. Biochem. Biophys. Res. Commun. 206: 266-271, 1995. 4. Lopez-Miranda, J.; Cruz, G.; Gomez, P.; Marin, C.; Paz, E.; Perez-Martinez, P.; Fuentes, F. J.; Ordovas, J. M.; Perez-Jimenez, F. : The influence of lipoprotein lipase gene variation on postprandial lipoprotein metabolism. J. Clin. Endocr. Metab. 89: 4721-4728, 2004.
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Gene Name
LPL
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Protein Name
Lipoprotein lipase(LPL)
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Gene Full Name
lipoprotein lipase
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Synonyms
EC 3.1.1 antibody|EC 3.1.1.34 antibody|HDLCQ11 antibody|LIPD antibody|LIPL_HUMAN antibody|Lipoprotein lipase antibody|LPL antibody|LPL protein antibody|MGC137861 antibody
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Uniprot ID
P06858
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
