This antibody has been succesfully tested for use in following methods: Western blot
Target of this antibody: LIMK2
Formulation of this antibody: 100 µg (0.5 mg/ml) peptide affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
Alternative names of antibody target: EC 184.108.40.206 , LIMK-2 , LIM domain kinase 2 , LIMK
Recommended usage: Western blotting (1-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 70 kDa LIMK2 protein. It does not cross-react with LIMK1.
Storage information: Shelf time: 12 months in temp.: -20 deg.C
Shipping conditions: gel pack
Antibody host: Rabbit
This antibody reacts with: Human, mouse, rat
Clonality of antibody: Polyclonal
Isotype: Rabbit IgG
Purification method: Affinity purified
Immunogene sequence: Synthetic peptide corresponding to the C-terminus of human LIMK2
How to handle this antibody: The antibody solution should be gently mixed before use.
Background information: LIM kinases (LIMK1 and LIMK2) are serine/threnine kinases that have two zinc finger motifs, known as LIM motifs in their N-terminal regulatory domain. LIM kinases are involved in actin cytoskeleton regulation throμgh Rho-family GTPases and downstream kinases PAKs and ROCK. PAK1 and ROCK phosphorylate LIMK1 and LIMK2, which increases the activity of the kinases. Activated LIM kinases inhibit the actin depolymerization activity of cofilin by phosphorylation at the N-terminus of Cofilin.
Form of antibody: Liquid
Concentration of antibody: 0.5 mg/ml
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
French translation anticorps