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Type
Conjugated Primary Antibody
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Conjugated with
Cy3
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Host organism
Rabbit (Oryctolagus cuniculus)
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Target Protein Peptide
IRAK1BP1
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Specificity
This antibody may exhibit a minor secondary cross-reactivity with SMPD5 due to a 63% contiguous sequence similarity over the immunogen range. IRAK1BP1 Cy3
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Modification
No modification has been applied to this antibody
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Modification site
None
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Clonality
Polyclonal Antibody
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Clone
Polyclonal Antibodies
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Concentration
1ug per 1ul
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Subcellular locations
Cytoplasm, Nucleus
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Antigen Source
KLH conjugated synthetic peptide derived from human IRAK1BP1.
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Gene ID
134728
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Swiss Prot
Q5VVH5
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Applications
IF(IHC-P)
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Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
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Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
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Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
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Background information
Component of the IRAK1-dependent TNFRSF1A signaling pathway that leads to NF-kappa-B activation and is required for cell survival. Acts by enhancing RELA transcriptional activity (By similarity).
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Purification method
Purified by Protein A.
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Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
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Excitation emission
512,550nm/570,615nm
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Synonyms
AIP70; SIMPL; Interleukin-1 receptor-associated kinase 1-binding protein 1; IRAK1-binding protein 1; IRAK1BP1
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Also known as
IRAK1BP1 Polyclonal Antibody Cy3
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Other name
Anti-IRAK1BP1 Polyclonal Cy3
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Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-IRAK1BP1 Cy3 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Polyclonal Antibodies antibody is well suited for fluorescent microscopy.
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Conjugation
cy3 conjugation kit
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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Gene target
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Gene symbol
IRAK1BP1
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Short name
Anti-IRAK1BP1 Cy3
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Technique
Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Isotype
Immunoglobulin G (IgG)
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Label
Cy3
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Alternative name
IRAK1BP1 Polyclonal Antibody, Cy3 Conjugated
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Alternative technique
polyclonals, antibodies
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Alternative to gene target
interleukin-1 receptor-associated kinase 1 binding protein 1, IRAK1BP1 and IDBG-93345 and ENSG00000146243 and 134728, protein binding, nuclei, Irak1bp1 and IDBG-188214 and ENSMUSG00000032251 and 65099, IRAK1BP1 and IDBG-630000 and ENSBTAG00000020825 and 782235
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