Anti-hydrogen exchanger 3 (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-8601R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  hydrogen exchanger 3
• Specificity
  This antibody reacts specifically with hydrogen exchanger 3
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  Extracellular
• Antigen Source
  KLH conjugated synthetic peptide derived from human NHE3
• Gene ID
  N/A
• Swiss Prot
  N/A
• Applications
  IF(IHC-P)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  NHE-3 are integral membrane proteins that are expressed in most mammalian tissues, where they regulate intracellular pH and cell volume. NHEs mediate the transport of hydrogen (H+) ions out of cells in exchange for extracellular sodium (Na+) ions. While NHE-1 is ubiquitously expressed, the NHE isoforms 2-8 have distinct tissue- and cell type-dependent expression and inhibitory characteristics. NHE-3 localizes to the apical membrane of renal proximal tubules where it is responsible for most of the sodium transport and fluid reabsorption. NHE-3 translocates to internal pools where it mediates natriuresis when blood pressure increases abruptly. NHE-3 is also expressed in the stomach and functions to protect the mucosa by secreting protons that diffuse into the mucous cells.
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  Na+/H+ exchanger 3; NHE 3; NHE-3; NHE3; SL9A3_HUMAN; SLC9A 3; Sodium; Slc9a3; Sodium/hydrogen exchanger 3; Sodium/hydrogen exchanger, apical epithelial; Solute carrier family 9 sodium/hydrogen exchanger, isoform 3; Solute carrier family 9 sodium/hydrogen exchanger, member 3; Solute carrier family 9 member 3; MGC126718; MGC126720.
• Also known as
  hydrogen exchanger 3 Polyclonal Antibody
• Other name
  Anti-hydrogen exchanger 3 Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  hydrogen   exchanger  
• Gene symbol
  SLC9A3P1, SLC9A3R1, SLC9A3R2
• Short name
  Anti-hydrogen exchanger 3
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  hydrogen exchanger 3 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:11074
• Gene
  SLC9A3P1
• Long gene name
  solute carrier family 9 member 3 pseudogene 1
• Synonyms gene
  • NHE3P
  • SLC9A3P
  • SLC9A3P4
• Synonyms gene name
  • solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 pseudogene
  • solute carrier family 9 (sodium/hydrogen exchanger), member 3 pseudogene
  • solute carrier family 9 (sodium/hydrogen exchanger), member 3 pseudogene 1
  • solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 pseudogene 4
  • solute carrier family 9 (sodium/hydrogen exchanger), member 3 pseudogene 4
  • solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 pseudogene 1
• Locus
  10q11.23
• Discovery year
  1996-03-13
• Entrez gene record
  6551
• Pubmed identfication
  • 8833252
  • 8199403
• VEGA ID
  OTTHUMG00000018226

Gene info

• Identity
  HGNC:11075
• Gene
  SLC9A3R1
• Long gene name
  SLC9A3 regulator 1
• Synonyms gene name
  • solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1
  • solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1
  • solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
  • solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
• Synonyms
  • NHERF
  • EBP50
  • NHERF1
• GenBank acession
  • AF015926
• Locus
  17q25.1
• Discovery year
  1999-02-26
• Entrez gene record
  9368
• Pubmed identfication
  • 9314537
  • 9430655
• Classification
  • PDZ domain containing
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000178863

Gene info

• Identity
  HGNC:11076
• Gene
  SLC9A3R2
• Long gene name
  SLC9A3 regulator 2
• Synonyms gene name
  • solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 2
  • solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 2
  • solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 2
  • solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 2
• Synonyms
  • SIP-1
  • TKA-1
  • NHERF-2
  • E3KARP
• GenBank acession
  • AF004900
• Locus
  16p13.3
• Discovery year
  1999-02-26
• Entrez gene record
  9351
• Pubmed identfication
  • 9054412
  • 9671706
• Classification
  • PDZ domain containing
• VEGA ID
  OTTHUMG00000176956

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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