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Products_type
Antibody
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Products_short_name
[Replication protein A 14 kDa subunit]
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Products_name_syn
[Replication factor A protein 3.RPA3; REPA3; RPA14]
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Other_names
[replication protein A 14 kDa subunit; Replication protein A 14 kDa subunit; replication protein A 14 kDa subunit; replication protein A3; Replication factor A protein 3; RF-A protein 3]
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Other_gene_names
[RPA3; RPA3; REPA3; RP-A p14; REPA3; RPA14; RP-A p14; RF-A protein 3]
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Clonality
Polyclonal
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Reactivity
Human
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Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
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Storage_stability
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
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Tested_application
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). Not yet tested in other applications.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The kilo Daltons subunit weight of Rabbit anti- protein A 14 subunit polyclonal Antibody compared to your protein ladder can be shifted a little due to electrophoresis effects. 1 kDa = 1000 g/mol protein
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Properties
If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Test
MyBioSource supplies antibodies that are for research of human proteins. Replication factor subunits and DNA replication proteins are found in DNA replication complexes like GINS, MCM,.. and replication initiators producing two identical replicas of DNA from one original DNA molecule. This process occurs in all living organisms and is the basis for biological inheritance. DNA is made up of a double helix of two strands, and each strand of the original DNA molecule gene serves as a template for the production of the complementary strand, a process referred to as semiconservative replication. Cellular proofreading and error-checking mechanisms ensure near perfect fidelity for DNA replication of genes.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Gene symbol
EMC9
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Short name
Rabbit anti- protein A 14 subunit polyclonal Antibody
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Technique
anti-Human, Polyclonal, Antibody, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
IgG
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody tohuman Replication protein A 14 kiloDalton functionnal sequence polyclonal (antibody to-)
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Alternative technique
polyclonals, antibodies, rabbit-anti
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