Sheep anti-human Factor VIII (FVIII:C), FITC-Conjugated Affinity-Purified IgG
#
-
Catalog numberMBS512054
-
Price:
-
Size100ug
-
-
Alternative name1Anti-Sheep anti-human Factor VIII (FVIII:C), FITC-Conjugated Affinity-Purified IgG
-
Alternative name2Ovine anti-human Factor VIII (FVIII:C), FITC-Conjugated Affinity-Purified IgG
-
Alternative name3Ovinae anti-Homo sapiens Factor VIII (FVIII:C), FITC-Conjugated Affinity-Purified IgG
-
Alternative name4Factor VIII:C
-
Alternative name5Factor VIII:C, human
-
Other namesfactor VIII; N/A
-
Gene nameN/A
-
Gene name synonimsN/A
-
Other gene namesFVIII; N/A; N/A; N/A
-
CategoryAntibodies
-
ClonalityPolyclonal
-
Immunoglobulin isotypeN/A
-
ClonePolyclonal antibody
-
Host organismSheep
-
Species reactivityN/A
-
Specificity and cross reactivityPrior to conjugation, this antibody was specific for FVIII as demonstrated by immunoelectrophoresis and ELISA.
-
Purification methodAffinity purified, FITC conjugated IgG. Vial containing mL of affinity-purified IgG conjugated to fluorescein isothiocyanate (FITC). Total protein is 0.1 mg.
-
Form AppearanceAPIgG-FITC conjugate as a clear yellow liquid.
-
ConcentrationAPIgG-FITC concentration is mg/mL, determined by absorbance using an extinction coefficient (E1%280) of 14.
-
Storage and shippingStore at 2 degree C to 8 degree C and protect from light.
-
Tested forN/A
-
Descriptionproductone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
-
AdvisoryIn order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
-
TestA high affinity purification column was use to purify anti- Factor VIII (FVIII:C), FITC-Conjugated -Purified IgG by MBS Polyclonals by chromatographic size exclusion. MBS Polyclonals supplies antibodies that are for research of human proteins.
-
Additional descriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
-
PropertiesThis MBS Polyclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Polyclonals FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
ConjugationAnti-FITC Antibody
-
AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
-
Gene target
-
Gene symbolCOX8A, G3BP1, F8, MARCHF8
-
Short nameanti- Factor VIII (FVIII:C), FITC-Conjugated -Purified IgG
-
Techniqueanti-Human, affinity, purified, anti-, anti, FITC, IgG, antibody to, antibodies, antibodies against human proteins, antibody Conjugates, Fluorescein, IgGs, purifications
-
HostSheep
-
IsotypeIgG, IgG
-
LabelFITC
-
SpeciesHuman, Humans, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
-
Alternative nameovine Antibody tohuman Factor VIII (FVIII:C), fluorecein-coupled protein-enriched Immunoglobulin G
-
Alternative techniquepure, antibodies, fluorescine, immunoglobulins
-
Gene info
-
Identity
-
Gene
-
Long gene namecytochrome c oxidase subunit 8A
-
Synonyms gene
-
Synonyms gene name
- cytochrome c oxidase subunit VIII
- cytochrome c oxidase subunit VIIIA (ubiquitous)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1989-06-30
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Mitochondrial complex IV: cytochrome c oxidase subunits
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameG3BP stress granule assembly factor 1
-
Synonyms gene name
- GTPase activating protein (SH3 domain) binding protein 1
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2006-10-25
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- RNA binding motif containing
- Endoribonucleases
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namecoagulation factor VIII
-
Synonyms gene
-
Synonyms gene name
- coagulation factor VIII, procoagulant component
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2001-06-22
-
Entrez gene record
-
Pubmed identfication
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene namemembrane associated ring-CH-type finger 8
-
Synonyms gene
-
Synonyms gene name
- c-mir, cellular modulator of immune recognition
- membrane-associated ring finger (C3HC4) 8
- membrane-associated ring finger (C3HC4) 8, E3 ubiquitin protein ligase
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2003-11-12
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Ring finger proteins
- Membrane associated ring-CH-type fingers
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
-
Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data