H3K36me1 polyclonal antibody
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Catalog number6817-25
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Price:
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Size25 μg
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Antibody TargetH3K36me1
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Target Alternative NameHistone H3
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Antibody TypePolyclonal
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Species ReactivitiesHuman.
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Immunogen SequenceKLH-conjugated synthetic peptide of Histone H3 containing monomethylated lysine 36.
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AccessionP68431
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Gene Id8350
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AppearanceColorless liquid
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FormLiquid
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FormulationIn PBS with 0.05% sodium azide and 0.05% ProClin 300.
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PurificationAffinity purified
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ApplicationWestern blot, IF, ELISA, ChIP, Dot blot.
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Positive ControlWestern blot: HeLa cells, IF: U2OS cells, ELISA: Antigen, ChIP: HeLa cells, Dot blot: Histone Peptides.
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Application And UsagesWB: 1:1000, ELISA: 1:100, Dot Blot: 1:20,000, IF: 1:500, ChIP: 5 µg/ChIP.
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DescriptionHistones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedVerified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedVerified
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ChIP VerifiedVerified
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Dot Blot VerifiedVerified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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GroupPolyclonals and antibodies
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AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translationanticorps
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Gene target
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Short nameH3K36me1 polyclonal antibody
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TechniquePolyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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IsotypeRabbit IgG
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Alternative nameH3K36me1 polyclonal (antibody to-)
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Alternative techniquepolyclonals, antibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data