EGLN1 Antibody
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Catalog number6151-100
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PricePlease ask
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Size100 μg
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Antibody TargetEGLN1
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Target Alternative NameEGLN1, DKFZp 61F179, ECYT3, HIFPH2, HPH2, PHD2, SM20, SM20, ZMYND6.
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat
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Immunogen SequenceRecombinant protein of human EGLN1
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AccessionQ9GZT9
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Gene Id54583
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AppearanceColorless liquid
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FormLiquid
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Concentration1 mg/ml
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Formulation100 µg of antibody in 100 µl PBS containing 0.02% sodium azide, 50% glycerol, pH 7.3
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PurificationAffinity purified
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ApplicationWestern blot, Immunohistochemistry
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Positive ControlWestern Blot: Various cell lysates
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Application And UsagesWestern blot: 1:500 – 1:2000, IHC: 1:50 – 1:200.
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DescriptionPHD1 (Egln2), PHD2 (Egln1), and PHD3 (Egln3) are members of the Egln family of proline hydroxylases. They function as oxygen sensors that catalyze the hydroxylation of HIF on prolines 564 and 402, initiating the first step of HIF degradation through the VHL/ubiquitin pathway. PHD1 is highly expressed in a wide array of tissues whereas PHD2 and PHD3 are expressed mainly in heart and skeletal muscle. The mRNA levels of PHD are upregulated by HIF through the hypoxia response element under low oxygen conditions. These three enzymes also exhibit different peptide specificity target proteins, PHD1 and PHD2 can hydroxylate both proline 402 and proline 564, but PHD3 can only hydroxylate proline 564. In addition to HIF, PHD enzymes have also has been shown to catalyze the hydroxylation of RNA polymerase subunits and myogenin.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolEGLN1
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Short nameEGLN1 Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameEGLN1 (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameegl-9 family hypoxia inducible factor 1
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Synonyms gene
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Synonyms gene name
- EGL nine (C.elegans) homolog 1
- egl nine homolog 1 (C. elegans)
- egl-9 family hypoxia-inducible factor 1
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2000-06-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Zinc fingers MYND-type
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data