anti-E2F5 Antibody
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Catalog number31-226
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PricePlease ask
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Size50 ug
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Gene infoE2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It is more homologous to E2F4, a family member, than to other members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB.
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Gene namesE2F5, E2F-5
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Antibodie s labelunlabelled serum
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PurityAntibody is purified by peptide affinity chromatography method.
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Ab raised inRabbit
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TypePolyclonal
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AntigenAntibody produced in rabbits immunized with a synthetic peptide corresponding a region of human E2F5.
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Immuno applicationELISA, Western Blot
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Antibody adviceE2F5 antibody can be used for detection of E2F5 by ELISA at 1:1562500. E2F5 antibody can be used for detection of E2F5 by western blot at 0.4 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
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Suspention pH azideAntibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
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Keep atFor short periods of storage (days) store at 4 grades C. For longer periods of storage, store E2F5 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
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Works withHuman
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Omim nr134142811
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Ncbi nrNP_001942
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KDa38 kDa
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Vial with antibodyLyophilized
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Quantity per ml1 mg/ml
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NCBI GENE nr1875
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GENE symbolE2F5
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Antigene nameE2F transcription factor 5, p130-binding
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SourceHomo sapiens
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Protein nrQ15329
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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PropertiesIf you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolE2F5, E2F5-DT
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Short nameanti-E2F5 Antibody
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TechniqueAntibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Alternative nameAntibody toE2F5 (antibody to-)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameE2F transcription factor 5
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Synonyms gene name
- E2F transcription factor 5, p130-binding
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GenBank acession
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Locus
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Discovery year1995-09-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- E2F transcription factors
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameE2F5 divergent transcript
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Locus
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Discovery year2020-12-21
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Entrez gene record
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RefSeq identity
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Classification
- Divergent transcripts
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
