Anti-Cyclin D1 (Polyclonal), ALEXA Fluor 594

• Catalog number
  GENTObs-0623R-A594
• Price
  Please ask
• Size
  100 microliters

• Type
  Conjugated Primary Antibody
• Conjugated with
  ALEXA FLUOR® 594
• Host organism
  Rabbit (Oryctolagus cuniculus)
• Target Protein Peptide
  Cyclin D1
• Specificity
  This antibody reacts specifically with Cyclin D1
• Modification
  No modification has been applied to this antibody
• Modification site
  None
• Clonality
  Polyclonal Antibody
• Clone
  Polyclonal Antibodies
• Concentration
  1ug per 1ul
• Subcellular locations
  Cytoplasm, Nucleus
• Antigen Source
  KLH conjugated synthetic peptide derived from mouse Cyclin D1
• Gene ID
  12443
• Swiss Prot
  P25322
• Applications
  IF(IHC-P), IF(ICC)
• Applications with corresponding dilutions
  IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
• Cross reactive species
  Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus), Goat
• Cross Reactive Species details
  No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
• Background information
  Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (By similarity).
• Purification method
  Purified by Protein A.
• Storage
  Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
• Excitation emission
  590nm/617nm
• Synonyms
  cD1; CycD1; Cyl-1; PRAD1; bcl-1; AI32739; G1/S-specific cyclin-D1; Ccnd1
• Also known as
  Cyclin D1 Polyclonal Antibody
• Other name
  Anti-Cyclin D1 Polyclonal
• Advisory
  Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  For facs or microscopy Alexa 1 conjugate.
• Conjugation
  Alexa Fluor, ALEXA FLUOR® 594
• Conjugated
  Alexa conjugate 1
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

• Gene target
  Cyclin  
• Gene symbol
  CCND1, CCNDBP1
• Short name
  Anti-Cyclin D1
• Technique
  Polyclonal, anti-, anti, antibody to, antibodies, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 594
• Alternative name
  Cyclin D1 Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:1582
• Gene
  CCND1
• Long gene name
  cyclin D1
• Synonyms gene
  • BCL1
  • D11S287E
  • PRAD1
• Synonyms gene name
  • cyclin D1 (PRAD1: parathyroid adenomatosis 1)
• Synonyms
  • U21B31
• Synonyms name
  • parathyroid adenomatosis 1
  • B-cell CLL/lymphoma 1
  • G1/S-specific cyclin D1
• GenBank acession
  • Z23022
• Locus
  11q13.3
• Discovery year
  1991-06-06
• Entrez gene record
  595
• Pubmed identfication
  • 1826542
  • 1833066
• RefSeq identity
  • NM_053056
• Classification
  • Cyclins
• VEGA ID
  OTTHUMG00000167877
• Locus Specific Databases
  • LRG_990

Gene info

• Identity
  HGNC:1587
• Gene
  CCNDBP1
• Long gene name
  cyclin D1 binding protein 1
• Synonyms gene name
  • cyclin D-type binding-protein 1
• Synonyms
  • DIP1
  • GCIP
  • HHM
• Synonyms name
  • D-type cyclin-interacting protein 1
  • MAID protein
  • grap2 and cyclin D interacting protein
• GenBank acession
  • AF082569
• Locus
  15q15.2
• Discovery year
  1998-10-02
• Entrez gene record
  23582
• Pubmed identfication
  • 10801854
• RefSeq identity
  • NM_012142
• VEGA ID
  OTTHUMG00000130703

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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