-
Gene info
RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.
-
Gene names
SISd, eoCP, SCYA5, RANTES, TCP228, D17S136E, SIS-delta, C-C motif chemokine 5, EoCP
-
Antibodie s label
Biotin
-
Purity
Anti-hRANTES specific antibody was purified by affinity chromatography and then biotinylated.
-
Ab raised in
Rabbit
-
Type
Polyclonal
-
Antigen
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hRANTES (human RANTES).
-
Immuno application
ELISA, Western Blot
-
Antibody advice
ELISA:To detect hRANTES by direct ELISA (using 100 μL/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 ng/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hRANTES.Sandwich:To detect hRANTES by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 ug/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human RANTES (XP-5257) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hRANTES. Western Blot:To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hRANTES is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
-
Keep at
RANTES antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
-
Works with
Human
-
Omim nr
6175077
-
Ncbi nr
P13501
-
Vial with antibody
Lyophilized
-
NCBI GENE nr
6352
-
GENE symbol
CCL5
-
Antigene name
chemokine (C-C motif) ligand 5
-
Source
Homo sapiens
-
Protein nr
P13501
-
-
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
-
Properties
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
-
French translation
anticorps
