anti-Calreticulin Antibody

• Catalog number
  32-125
• Price
  Please ask
• Size
  0.1 mL

• Gene info
  Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.
• Gene names
  CRTC, CRP55, ERp60, RO, CRT, SSA, cC1qR, HEL-S-99n
• Antibodie s label
  unlabelled serum
• Ab raised in
  Rabbit
• Type
  Polyclonal
• Antigen
  Synthetic peptide corresponding to aa (E-E-E-D-V-P-G-Q-A-K-D-E-L-C)of human Calreticulin
• Immuno application
  ELISA, Western Blot
• Antibody advice
  Western Blot:1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.
• Suspention pH azide
  Rabbit anti-serum.
• Keep at
  Calreticulin antibody can be stored at -20 grades C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Works with
  Human
• Omim nr
  117501
• Ncbi nr
  P27797
• NCBI GENE nr
  811
• GENE symbol
  CALR
• Antigene name
  calreticulin
• Source
  Homo sapiens
• Protein nr
  P27797
• Picture
• Pdf tech file
• More antibodies
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Calreticulin  
• Gene symbol
  CALR4P, CALR, CALR3
• Short name
  anti-Calreticulin Antibody
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Alternative name
  Antibody toCalreticulin (antibody to-)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:35456
• Gene
  CALR4P
• Long gene name
  calreticulin 4, pseudogene
• Locus
  1p32.3
• Discovery year
  2010-03-12
• Entrez gene record
  441884
• Pubmed identfication
  • 20210993
• VEGA ID
  OTTHUMG00000008233

Gene info

• Identity
  HGNC:1455
• Gene
  CALR
• Long gene name
  calreticulin
• Synonyms
  • RO
  • SSA
  • cC1qR
  • CRT
  • FLJ26680
• Synonyms name
  • Sicca syndrome antigen A (autoantigen Ro; calreticulin)
  • autoantigen Ro
• GenBank acession
  • M84739
• Locus
  19p13.13
• Discovery year
  1990-09-10
• Entrez gene record
  811
• Pubmed identfication
  • 2365822
• RefSeq identity
  • NM_004343
• Classification
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000180574
• Locus Specific Databases
  • LRG_828

Gene info

• Identity
  HGNC:20407
• Gene
  CALR3
• Long gene name
  calreticulin 3
• Synonyms
  • CRT2
  • FLJ25355
  • MGC26577
  • CT93
• Synonyms name
  • cancer/testis antigen 93
  • calsperin
• GenBank acession
  • AK058084
• Locus
  19p13.11
• Discovery year
  2003-02-04
• Entrez gene record
  125972
• Pubmed identfication
  • 12384296
• RefSeq identity
  • NM_145046
• VEGA ID
  OTTHUMG00000152571
• Locus Specific Databases
  • LRG_422

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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