anti-Bub1 Antibody

• Catalog number
  4229
• Price
  Please ask
• Size
  0.02 mg, 0.1 mg

• Gene info
  Bub1 Antibody: Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. It is thought to be required for assembly of a functional inner centromere, sister chromatid cohesion via targeting of the Shugoshim protein and metaphase congression. Bub1 functions by phosphorylating cdc20, a member of the mitotic checkpoint complex and activating the spindle checkpoint. A related protein kinase Bub3 interacts with Bub1 and targets it to kinetochores prior to chromosome alignment. Mutations in bub1 have been associated with aneuploidy and several forms of cancer.
• Gene names
  Bub1 Antibody: BUB1A, BUB1L, hBUB1, BUB1A
• Antibodie s label
  unlabelled serum
• Purity
  Bub1 Antibody is affinity chromatography purified via peptide column.
• Ab raised in
  Rabbit
• Type
  Polyclonal
• Immunoglobulin
  IgG
• Antigen
  Bub1 antibody was made against a 19 amino acid synthetic peptide from near the amino terminus of human Bub1.
• Immuno application
  ELISA, Western Blot, immuno-cyto-chemistry, immuno fluorescence
• Antibody advice
  Bub1 antibody can be used for detection of bub1 by Western blot at 2 - 4 ug/mL. Antibody can also be used for immunocytochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL.
• Suspention pH azide
  Bub1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Keep at
  Bub1 antibody can be stored at 4 grades C for three months and -20 grades C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Works with
  Human, Mouse
• Omim nr
  8134347
• Ncbi nr
  O43683
• Vial with antibody
  in solution
• Quantity per ml
  1 mg/mL
• NCBI GENE nr
  699
• GENE symbol
  BUB1
• Antigene name
  budding uninhibited by benzimidazoles 1 homolog (yeast)
• Source
  Homo sapiens
• Protein nr
  O43683
• Picture
• Pdf tech file
• More antibodies
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
• Properties
  If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Bub1  
• Gene symbol
  BUB1, BUB1P1, BUB1B
• Short name
  anti-Bub1 Antibody
• Technique
  Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
• Alternative name
  Antibody toBub1 (antibody to-)
• Alternative technique
  antibodies
• Alternative to gene target
  budding uninhibited by benzimidazoles 1 homolog (yeast), BUB1A and BUB1L and hBUB1, BUB1 and IDBG-65670 and ENSG00000169679 and 699, transferase activity, nuclei, Bub1 and IDBG-205152 and ENSMUSG00000027379 and 12235, BUB1 and IDBG-628990 and ENSBTAG00000021181 and 514777

Gene info

• Identity
  HGNC:1148
• Gene
  BUB1
• Long gene name
  BUB1 mitotic checkpoint serine/threonine kinase
• Synonyms gene
  • BUB1L
• Synonyms gene name
  • budding uninhibited by benzimidazoles 1 (yeast homolog)
  • budding uninhibited by benzimidazoles 1 homolog (yeast)
• Synonyms
  • hBUB1
  • BUB1A
• GenBank acession
  • AF046078
• Locus
  2q13
• Discovery year
  1997-08-18
• Entrez gene record
  699
• Pubmed identfication
  • 9441741
• RefSeq identity
  • NM_004336
• VEGA ID
  OTTHUMG00000153638

Gene info

• Identity
  HGNC:49529
• Gene
  BUB1P1
• Long gene name
  BUB1 mitotic checkpoint serine/threonine kinase pseudogene 1
• Locus
  10q26.2
• Discovery year
  2014-01-08
• Entrez gene record
  100422363
• RefSeq identity
  • NG_025548
• VEGA ID
  OTTHUMG00000184638

Gene info

• Identity
  HGNC:1149
• Gene
  BUB1B
• Long gene name
  BUB1 mitotic checkpoint serine/threonine kinase B
• Synonyms gene name
  • budding uninhibited by benzimidazoles 1 (yeast homolog), beta
  • budding uninhibited by benzimidazoles 1 homolog beta (yeast)
• Synonyms
  • BUBR1
  • MAD3L
  • Bub1A
  • SSK1
• GenBank acession
  • AF107297
• Locus
  15q15.1
• Discovery year
  1998-03-25
• Entrez gene record
  701
• Pubmed identfication
  • 9889005
• VEGA ID
  OTTHUMG00000129877
• Locus Specific Databases
  • LRG_489

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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